A Sensitive Radioimmunoassay of Parathyroid Hormone in Human Serum Using a Specific Extraction Procedure
Abstract
A radioimmunoassay of human parathroid hormone (PTH) is described. PTH was extracted from serum by adsorption to and elution from microfine silica, causing a 3.2-fold greater hormone concentration in extract than in serum. The extraction procedure eliminated from the assay unspecific interference caused by serum factors. The detection limit was 10 pg bovine PTH. The concentration of PTH was measurable in serum from 95% of normal subjects (mean, 68 pg/ml; SD., 18), was undetectable in sera from hypoparathyroid patients, and elevated in 96% of sera from patients with primary hyperparathyroidism. The presented data suggest that the assay mainly measures PTH(1-84).
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Such interference has been recognised in immunoassays for cholecystokinin, parathyroid hormone, thyrotropic hormone, glucagon, secretin, angiotensin, growth hormone, vasoactive intestinal peptide, gonadotrophins and gonadotrophin-releasing hormone, all of which were resolved by sample extraction.
“Such interference has also been recognised in immunoassays for cholecystokinin 80, parathyroid hormone 81, thyrotropic hormone 82, glucagon 83, secretin 84, 85, angiotensin 86, growth hormone 87, vasoactive intestinal peptide 88, gonadotrophins 89 and gonadotrophin‐releasing hormone 90, all of which were resolved by sample extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [81]
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- Authors
- Christensen, M. S.
- Affiliations
- Aarhus Municipality (DK)
- Corresponding author
- Christensen, Merete Sanvig
- Venue
- Scandinavian Journal of Clinical and Laboratory Investigation
- Publisher
- Informa UK Limited
- Published
- 1976-01
- Volume
- 36
- Issue
- 4
- Pages
- 313-322
- ISSN
- 0036-5513 (ISSN-L)
- 1502-7686
- OpenAlex
- W2150443814
- Cited by
- 49
- References
- 20
- Citations by year
- 2016: 1
- FWCI
- 13.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Scandinavian Journal of Clinical and Laboratory Investigation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- Topics
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Surgery › Thyroid and Parathyroid Surgery
- Keywords
- parathyroid hormone, radioimmunoassay, hypoparathyroidism, primary hyperparathyroidism, PTH(1-84)
- MeSH terms
- Adsorption, Humans, Hyperparathyroidism, Hypoparathyroidism, Parathyroid Glands, Parathyroid Hormone, Radioimmunoassay, Time Factors
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