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Cited by 502

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Gliadin, zonulin and gut permeability: Effects on celiac and non-celiac intestinal mucosa and intestinal cell lines

Open access
Drago, Sandro, El Asmar, Ramzi, Di Pierro, Mariarosaria, Grazia Clemente, Maria, Sapone, Amit Tripathi Anna, Thakar, Manjusha, Iacono, Giuseppe, Carroccio, Antonio, D'Agate, Cinzia, Not, Tarcisio, Zampini, Lucia, Catassi, Carlo, Fasano, Alessio·2006·Scandinavian Journal of Gastroenterology
DOI 10.1080/00365520500235334
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Abstract

OBJECTIVE: Little is known about the interaction of gliadin with intestinal epithelial cells and the mechanism(s) through which gliadin crosses the intestinal epithelial barrier. We investigated whether gliadin has any immediate effect on zonulin release and signaling. MATERIAL AND METHODS: Both ex vivo human small intestines and intestinal cell monolayers were exposed to gliadin, and zonulin release and changes in paracellular permeability were monitored in the presence and absence of zonulin antagonism. Zonulin binding, cytoskeletal rearrangement, and zonula occludens-1 (ZO-1) redistribution were evaluated by immunofluorescence microscopy. Tight junction occludin and ZO-1 gene expression was evaluated by real-time polymerase chain reaction (PCR). RESULTS: When exposed to gliadin, zonulin receptor-positive IEC6 and Caco2 cells released zonulin in the cell medium with subsequent zonulin binding to the cell surface, rearrangement of the cell cytoskeleton, loss of occludin-ZO1 protein-protein interaction, and increased monolayer permeability. Pretreatment with the zonulin antagonist FZI/0 blocked these changes without affecting zonulin release. When exposed to luminal gliadin, intestinal biopsies from celiac patients in remission expressed a sustained luminal zonulin release and increase in intestinal permeability that was blocked by FZI/0 pretreatment. Conversely, biopsies from non-celiac patients demonstrated a limited, transient zonulin release which was paralleled by an increase in intestinal permeability that never reached the level of permeability seen in celiac disease (CD) tissues. Chronic gliadin exposure caused down-regulation of both ZO-1 and occludin gene expression. CONCLUSIONS: Based on our results, we concluded that gliadin activates zonulin signaling irrespective of the genetic expression of autoimmunity, leading to increased intestinal permeability to macromolecules.

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Crossref · 10.1080/00365520500235334

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Metadata

Authors
Drago, S., El Asmar, R., Di Pierro, M., Grazia Clemente, M., Sapone, A. T. A., Thakar, M., Iacono, G., Carroccio, A., D'Agate, C., Not, T., Zampini, L., Catassi, C., & Fasano, A.
Venue
Scandinavian Journal of Gastroenterology
Publisher
Informa UK Limited
Published
2006-01
Volume
41
Issue
4
Pages
408-419
ISSN
  • 0036-5521 (ISSN-L)
  • 1502-7708
DOI
10.1080/00365520500235334
OpenAlex
W2097319034
Cited by
502
References
38
FWCI
9.45
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Gliadin, zonulin and gut permeability: Effects on celiac and non-celiac intestinal mucosa and intestinal cell lines. https://app.cochat.ai/papers/10.1080/00365520500235334
The paper
Drago, S., El Asmar, R., Di Pierro, M., Grazia Clemente, M., Sapone, A. T. A., Thakar, M., Iacono, G., Carroccio, A., D'Agate, C., Not, T., Zampini, L., Catassi, C., & Fasano, A. (2006). Gliadin, zonulin and gut permeability: Effects on celiac and non-celiac intestinal mucosa and intestinal cell lines. Scandinavian Journal of Gastroenterology. https://doi.org/10.1080/00365520500235334

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.