Cited by 20Enzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationFungal and yeast genetics research

Molecular modification and biotechnological applications of microbial aspartic proteases

Herman, Richard Ansah, Ayepa, Ellen, Zhang, Wen-Xin, Li, Zong-Nan, Zhu, Xuan, Ackah, Michael, Yuan, Shuang-Shuang, You, Shuai +1 more·2024·Critical Reviews in Biotechnology

Abstract

The growing preference for incorporating microbial aspartic proteases in industries is due to their high catalytic function and high degree of substrate selectivity. These properties, however, are attributable to molecular alterations in their structure and a variety of other characteristics. Molecular tools, functional genomics, and genome editing technologies coupled with other biotechnological approaches have aided in improving the potential of industrially important microbial proteases by addressing some of their major limitations, such as: low catalytic efficiency, low conversion rates, low thermostability, and less enzyme yield. However, the native folding within their full domain is dependent on a surrounding structure which challenges their functionality in substrate conversion, mainly due to their mutual interactions in the context of complex systems. Hence, manipulating their structure and controlling their expression systems could potentially produce enzymes with high selectivity and catalytic functions. The proteins produced by microbial aspartic proteases are industrially capable and far-reaching in regulating certain harmful distinctive industrial processes and the benefits of being eco-friendly. This review provides: an update on current trends and gaps in microbial protease biotechnology, exploring the relevant recombinant strategies and molecular technologies widely used in expression platforms for engineering microbial aspartic proteases, as well as their potential industrial and biotechnological applications.

Integrity signals

Registry

Crossref · 10.1080/07388551.2023.2171850

Cites

158 of 158 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

1 retracted

Flagged first, then by citations

1 of the 159 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Herman, R. A., Ayepa, E., Zhang, W.-X., Li, Z.-N., Zhu, X., Ackah, M., Yuan, S.-S., You, S., & Wang, J.
Affiliations
  • Jiangsu University of Science and Technology (CN)
  • Council for Scientific and Industrial Research (GH)
  • Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, P.R. China
Corresponding author
Wang, Jun
Venue
Critical Reviews in Biotechnology
Published
2023-02-26
Volume
44
Issue
3
Pages
388-413
ISSN
  • 0738-8551 (ISSN-L)
  • 1549-7801
OpenAlex
W4322183410
Cited by
20
References
159
Citations by year
2026: 7 · 2025: 6 · 2024: 5 · 2023: 2
FWCI
0.60
Type
journal article
Language
en
Open access
closed
Locations
  • Critical Reviews in Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Jiangsu Agricultural Science and Technology Innovation Fund (CX(20)2029)
  • Key Research and Development Program (Modern Agriculture) of Jiangsu Province (BE2019358)
  • Jiangsu Excellent Postdoctoral Project Fund
  • Jiangsu University of Science and Technology Postdoctoral Research Fund (1062152103)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
aspartic protease, microbial proteases, enzyme engineering, recombinant protein expression, thermal stability, catalytic efficiency, substrate specificity, genome editing, functional genomics, industrial enzymes
MeSH terms
Biotechnology, Peptide Hydrolases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review