Molecular modification and biotechnological applications of microbial aspartic proteases
Abstract
The growing preference for incorporating microbial aspartic proteases in industries is due to their high catalytic function and high degree of substrate selectivity. These properties, however, are attributable to molecular alterations in their structure and a variety of other characteristics. Molecular tools, functional genomics, and genome editing technologies coupled with other biotechnological approaches have aided in improving the potential of industrially important microbial proteases by addressing some of their major limitations, such as: low catalytic efficiency, low conversion rates, low thermostability, and less enzyme yield. However, the native folding within their full domain is dependent on a surrounding structure which challenges their functionality in substrate conversion, mainly due to their mutual interactions in the context of complex systems. Hence, manipulating their structure and controlling their expression systems could potentially produce enzymes with high selectivity and catalytic functions. The proteins produced by microbial aspartic proteases are industrially capable and far-reaching in regulating certain harmful distinctive industrial processes and the benefits of being eco-friendly. This review provides: an update on current trends and gaps in microbial protease biotechnology, exploring the relevant recombinant strategies and molecular technologies widely used in expression platforms for engineering microbial aspartic proteases, as well as their potential industrial and biotechnological applications.
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Microbial aspartic proteases can break the peptide bonds between hydrophobic residues, allowing for the production of cheese with the desired composition and texture.
“Microbial aspartic proteases can break the peptide bonds between hydrophobic residues.This allows for the production of cheese with the desired composition and texture (Herman et al., 2024;Lombardi et al., 2018).”
From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Herman et al., 2024
Mucor spp. and M. circinelloides have the potential to replace chymotrypsin in cheese production.
“Mucor spp.and M. circinelloides have the potential to replace chymotrypsin in cheese production.”
From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Herman et al., 2024
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- Authors
- Herman, R. A., Ayepa, E., Zhang, W.-X., Li, Z.-N., Zhu, X., Ackah, M., Yuan, S.-S., You, S., & Wang, J.
- Affiliations
- Jiangsu University of Science and Technology (CN)
- Council for Scientific and Industrial Research (GH)
- Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, P.R. China
- Corresponding author
- Wang, Jun
- Venue
- Critical Reviews in Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2023-02-26
- Volume
- 44
- Issue
- 3
- Pages
- 388-413
- ISSN
- 0738-8551 (ISSN-L)
- 1549-7801
- OpenAlex
- W4322183410
- Cited by
- 20
- References
- 159
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 5 · 2023: 2
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Critical Reviews in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Jiangsu Agricultural Science and Technology Innovation Fund (CX(20)2029)
- Key Research and Development Program (Modern Agriculture) of Jiangsu Province (BE2019358)
- Jiangsu Excellent Postdoctoral Project Fund
- Jiangsu University of Science and Technology Postdoctoral Research Fund (1062152103)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- aspartic protease, microbial proteases, enzyme engineering, recombinant protein expression, thermal stability, catalytic efficiency, substrate specificity, genome editing, functional genomics, industrial enzymes
- MeSH terms
- Biotechnology, Peptide Hydrolases
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