Susceptibility of LDL to oxidation in vitro and antioxidant capacity in familial combined hyperlipidemia: comparison of patients with different lipid phenotypes
Open accessAbstract
BACKGROUND: There is increasing evidence that oxidation of low-density lipoprotein (LDL) plays an important role in atherogenesis. AIM: To explore the LDL oxidizability and its determinants in familial combined hyperlipidemia (FCHL) patients with different phenotypes. METHOD: The study included 59 FCHL family members with different lipid phenotypes, 39 non-affected relatives, and 30 spouses as healthy controls. RESULTS: The lag time for LDL oxidation was significantly shorter in FCHL patients with different lipid phenotypes as compared to healthy controls. There were no significant differences in the propagation rate and conjugated diene formation and alpha-tocopherol content in LDL between the FCHL groups and healthy controls. Plasma concentrations of alpha-tocopherol in all FCHL patients and uric acid in FCHL patients with IIB and IV phenotypes were significantly higher than in healthy controls. Plasma total peroxyl radical trapping capacity measured (TRAPmea) and TRAPcalc tended to be higher in affected FCHL groups, but the difference was significant only for IIB phenotype. The peak LDL particle size in the combined group of FCHL patients was significantly smaller than in healthy controls. The lag time for LDL oxidation correlated significantly with LDL size both in the group of FCHL family members (r = 0.477, P<0.001) and in the healthy controls (r = 0.482, P<0.01). CONCLUSIONS: LDL from FCHL patients irrespectively of lipid phenotypes is more susceptible to oxidation in vitro than LDL from healthy controls. This increased susceptibility of LDL to oxidation in vitro seems to be attributed to the abundance of small dense LDL particles and not to the defect of antioxidant capacity in FCHL
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Possible mechanisms connecting small LDL particles to vascular events include greater oxidizability and increased passage through the endothelial barrier.
“Potential mechanisms that link small LDL particles to increased risk of vascular events include augmented oxidizability (36) and increased permeability through the endothelial barrier (37).”
From Alternate day fasting and endurance exercise combine to reduce body weight and favorably alter plasma lipids in obese humans (2013) · cites this paper as [36]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Ellman, G. L. (1959) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(59)90090-6 · Cited by 26,824 · see citing works
Havel, R. J., Eder, H. A., & Bragdon, J. H. (1955) · Journal of Clinical Investigation · doi:10.1172/jci103182 · Cited by 8,756 · see citing works
Ames, B. N., Cathcart, R., Schwiers, E., & Hochstein, P. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.11.6858 · Cited by 2,915 · see citing works
Continuous Monitoring ofin VztroOxidation of Human Low Density Lipoprotein
Esterbauer, H., Striegl, G., Puhl, H., & Rotheneder, M. (1989) · Free Radical Research Communications · doi:10.3109/10715768909073429 · Cited by 1,790 · see citing works
Goldstein, J. L., Schrott, H. G., Hazzard, W. R., Bierman, E. L., & Motulsky, A. G. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107332 · Cited by 1,444 · see citing works
The oxidation hypothesis of atherosclerosis
Witztum, J. L. (1994) · The Lancet · doi:10.1016/s0140-6736(94)92346-9 · Cited by 1,394 · see citing works
Susceptibility to low-density lipoprotein oxidation and coronary atherosclerosis in man
Regnström, J., Nilsson, J., Tornvall, P., Hamsten, A., & Landou, C. (1992) · The Lancet · doi:10.1016/0140-6736(92)91129-v · Cited by 574 · see citing works
Chait, A., Brazg, R., Tribble, D. L., & Krauss, R. M. (1993) · The American Journal of Medicine · doi:10.1016/0002-9343(93)90144-e · Cited by 554 · see citing works
Coresh, J., Kwiterovich, P., Smith, H., & Bachorik, P. S. (1993) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)35731-x · Cited by 290 · see citing works
Effect of Antioxidants on Oxidative Modification of LDL
Esterbauer, H., Puhl, H., Dieber‐Rotheneder, M., Waeg, G., & Rabl, H. (1991) · Annals of Medicine · doi:10.3109/07853899109150520 · Cited by 261 · see citing works
Dejager, S., Bruckert, E., & Chapman, M. (1993) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40756-4 · Cited by 237 · see citing works
Kw, D., & Ef, B. (1961) · PubMed · Cited by 233 · see citing works
Spectrophotometric assay for total peroxyl radical-trapping antioxidant potential in human serum
Valkonen, M. M., & Kuusi, T. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37249-7 · Cited by 180 · see citing works
Self-citationAtherogenic, dense low-density lipoproteins. Pathophysiology and new therapeutic approaches.
Chapman, M. J., Guérin, M., & Bruckert, É. (1998) · PubMed · Cited by 178 · see citing works
Characterization of low-density lipoprotein subclasses
Austin, M. A., Hokanson, J. E., & Brunzell, J. D. (1994) · Current Opinion in Lipidology · doi:10.1097/00041433-199412000-00002 · Cited by 172 · see citing works
McNamara, J. R., Small, D. M., Li, Z., & Schaefer, E. J. (1996) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37557-x · Cited by 147 · see citing works
Kashyap, M. L., Hynd, B. A., & Robinson, K. (1980) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39803-5 · Cited by 127 · see citing works
LDL Size and Risk of Coronary Heart Disease in Elderly Men and Women
Mykkänen, L. M., Kuusisto, J., Haffner, S. M., Laakso, M., & Austin, M. A. (1999) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.19.11.2742 · Cited by 112 · see citing works
Lahdenper, S., Syv�nne, M., Kahri, J., & Taskinen, M. (1996) · Diabetologia · doi:10.1007/bf00400677 · Cited by 103 · see citing works
Self-citationPlasma triglyceride and LDL heterogeneity in familial combined hyperlipidemia.
Hokanson, J. E., Austin, M. A., Zambon, A., & Brunzell, J. D. (1993) · Arteriosclerosis and Thrombosis A Journal of Vascular Biology · doi:10.1161/01.atv.13.3.427 · Cited by 101 · see citing works
Patel, R. P., Svistunenko, D. A., Wilson, M. T., et al. (1997) · Biochemical Journal · doi:10.1042/bj3220425 · Cited by 93 · see citing works
LDL physical and chemical properties in familial combined hyperlipidemia
Hokanson, J. E., Krauss, R. M., Albers, J. J., Austin, M. A., & Brunzell, J. D. (1995) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.15.4.452 · Cited by 76 · see citing works
Pleiotropic Genetic Effects on LDL Size, Plasma Triglyceride, and HDL Cholesterol in Families
Edwards, K. L., Mahaney, M. C., Motulsky, A. G., & Austin, M. A. (1999) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.19.10.2456 · Cited by 75 · see citing works
Bredie, S. J. H., Kiemeney, L. A. L. M., de Haan, A. F., Demacker, P. N. M., & Stalenhoef, A. F. H. (1996) · PubMed Central · Cited by 70 · see citing works
INCREASED LEUCOCYTE OXIDATIVE METABOLISM IN HYPERLIPOPROTEINAEMIA
Ludwig, P., Hunninghake, D. B., & Hoidal, J. R. (1982) · The Lancet · doi:10.1016/s0140-6736(82)90546-3 · Cited by 67 · see citing works
Allayee, H., Aouizerat, B. E., Cantor, R. M., Dallinga‐Thie, G. M., Krauss, R. M., Lanning, C. D., Rotter, J. I., Lusis, A. J., & de Bruin, T. W. (1998) · The American Journal of Human Genetics · doi:10.1086/301983 · Cited by 66 · see citing works
Phenotype expression in familial combined hyperlipidemia
Porkka, K. V. K., Nuotio, I. O., Pajukanta, P., Ehnholm, C., Suurinkeroinen, L., Syvänne, M., Lehtimäki, T. J., Lahdenkari, A.-T., Lahdenperä, S., Ylitalo, K., et al. (1997) · Atherosclerosis · doi:10.1016/s0021-9150(97)00134-2 · Cited by 61 · see citing works
Self-citationClaudio Napoli, A., Postiglione, A., Triggiani, M., Corso, G., Palumbo, G. A., Carbone, V., Ruocco, A., Ambrosio, G., Montefusco, S., Malorni, A., et al. (1995) · Atherosclerosis · doi:10.1016/0021-9150(95)05612-2 · Cited by 59 · see citing works
Quantitation of Free, Total, and Antibody-bound Insulin in Insulin-Treated Diabetics
Gennaro, W. D., & Norman, J. D. V. (1975) · Clinical Chemistry · doi:10.1093/clinchem/21.7.873 · Cited by 54 · see citing works
Prónai, L., Hiramatsu, K., Saigusa, Y., et al. (1991) · Atherosclerosis · doi:10.1016/0021-9150(91)90242-u · Cited by 54 · see citing works
The role of α-tocopherol as a peroxyl radical scavenger in human low density lipoprotein
Smith, D. R., O'leary, V. J., & Darley-Usmar, V. M. (1993) · Biochemical Pharmacology · doi:10.1016/0006-2952(93)90189-4 · Cited by 43 · see citing works
The Redox Status of Coenzyme Q10 in Total LDL as an Indicator of In Vivo Oxidative Modification
de Rijke, Y. B., Bredie, S. J. H., Demacker, P. N. M., et al. (1997) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.17.1.127 · Cited by 37 · see citing works
Cominacini, L., Pastorino, A. M., Garbin, U., Campagnola, M., de Santis, A., Davoli, A., Faccini, G., Bertozzo, L., Pasini, F., & Af, P. (1994) · PubMed · Cited by 27 · see citing works
Ayaori, M., Ishikawa, T., Yoshida, H., et al. (1997) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.17.11.2540 · Cited by 26 · see citing works
Juo, S. H., Bredie, S. J. H., Kiemeney, L. A. L. M., Demacker, P. N. M., & Stalenhoef, A. F. H. (1998) · The American Journal of Human Genetics · doi:10.1086/301962 · Cited by 25 · see citing works
Visioli, F., Bordone, R. P., PERUGINI, C., et al. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.2212 · Cited by 19 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- Bredie SJH, Demacker PNM, Stalenhoef AFH. Metabolic 3. and genetic aspects of familial combined hyperlipidaemia with emphasis on low-density lipoprotein heterogeneity. Eur J Clin Invest 1997; 27: 802±11.
- Liu ML, Bergholm R, Makimattila S, Lahdenpera S, Valkonen M, Hilden H, et al. A marathon run increases the susceptibility of LDL to oxidation in vitro and modi®es plasma antioxidants. Am J Physiol 1999; 276: E1083±91.
- Esterbauer H, Striegl G, Puhl H, Rotheneder M. Continuous monitoring of in vitro oxidation of human low density lipo- protein. Free Radic Res Commun 1989; 6: 67±75.
Cited by
Sorted by citations
Bhutani, S., Klempel, M. C., Kroeger, C. M., Trepanowski, J. F., & Varady, K. A. (2013) · Obesity · doi:10.1002/oby.20353 · Cited by 349 · see citing works
Calabresi, L., Villa, B., Canavesi, M., Sirtori, C. R., James, R. W., Bernini, F., & Franceschini, G. (2004) · Metabolism · doi:10.1016/j.metabol.2003.09.007 · Cited by 132 · see citing works
Varady, K. A., Bhutani, S., Klempel, M. C., & Kroeger, C. M. (2011) · Lipids in Health and Disease · doi:10.1186/1476-511x-10-119 · Cited by 116 · see citing works
- Authors
- Liu, M.-L., Ylitalo, K., Vakkilainen, J., Nuotio, I., Valkonen, M., Lahdenperä, S., Viikari, J., & Taskinen, M.-R.
- Affiliations
- Helsinki University Hospital (FI)
- Turku University Hospital (FI)
- Venue
- Annals of Medicine
- Publisher
- Informa UK Limited
- Published
- 2002-01
- Volume
- 34
- Issue
- 1
- Pages
- 48-54
- ISSN
- 0785-3890 (ISSN-L)
- 1365-2060
- OpenAlex
- W1972533171
- Cited by
- 23
- References
- 36
- Citations by year
- 2024: 2 · 2020: 1 · 2018: 2 · 2015: 3 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Annals of Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Helsingin ja Uudenmaan Sairaanhoitopiiri
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Paraoxonase enzyme and polymorphisms
- Keywords
- LDL oxidation, familial combined hyperlipidemia, lipid phenotypes, antioxidant capacity, alpha-tocopherol, uric acid, small dense LDL, atherogenesis, conjugated dienes
- MeSH terms
- Adult, Analysis of Variance, Antioxidants, Arteriosclerosis, Female, Humans, Hyperlipidemia, Familial Combined, Lipoproteins, LDL, Male, Middle Aged, Oxidation-Reduction, Particle Size, Phenotype, Probability, Reference Values, Lipid Peroxidation, Cohort Studies, Case-Control Studies
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
