Inactivation of individual mammalian cells by single alpha-particles
Abstract
PURPOSE: To measure clonogenic death of Chinese hamster V79 cells following exposure to a defined number of 4.3 MeV alpha-particles (track-averaged LET = 105 keV/micron). MATERIALS AND METHODS: Cells were irradiated at the radiobiological facility installed at the TTT-3 Tandem accelerator in Naples by using a 'Biostack' approach, which allows the positions of incident tracks relative to cells to be carefully determined. Subcellular structure was identified by fluorescence microscopy, while tracks were visualized by LR-115 solid state nuclear track detectors. RESULTS: Particle hits in the cytoplasm did not significantly affect cell survival, yet survival probability decreased exponentially as a function of the number of nuclear traversals. Measured probability of surviving to exactly one 4.3 MeV alpha-particle traversal in the cell nucleus was 0.67 +/- 0.10. Inactivation cross-section was substantially higher than expected from conventional survival curves. However, folding of the data with Poisson statistics showed that survival level expected if a mean of one alpha-particle goes through a nucleus is higher than the measured value after exactly one particle traversal. CONCLUSIONS: V79 cells have about 67% probability to survive a single alpha-particle traversal in the cell nucleus. Single-particle survival curves are consistent with conventional dose-survival relationships, once Poisson distribution of traversals is taken into account.
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A single alpha-particle traversal of a cell nucleus can cause multiple double-strand breaks and likely lead to cell death.
“Consequently, a single a -particle traversal of a cell nucleus can cause multiple double-strand breaks and likely lead to cell death ( 22 ).”
From Normal-Tissue Tolerance to Radiopharmaceutical Therapies, the Knowns and the Unknowns (2021) · cites this paper as [22]
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- Authors
- PUGLIESE, M. D.
- Venue
- International Journal of Radiation Biology
- Publisher
- Informa UK Limited
- Published
- 1997-01
- Volume
- 72
- Issue
- 4
- Pages
- 397-407
- ISSN
- 0955-3002 (ISSN-L)
- 1362-3095
- OpenAlex
- W2141003974
- Cited by
- 45
- References
- 22
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2018: 1 · 2017: 2 · 2015: 2 · 2014: 1 · 2012: 3
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Radiation Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Physical Sciences › Physics and Astronomy › Nuclear and High Energy Physics › Particle Detector Development and Performance
- Keywords
- clonogenic survival, V79 cells, inactivation cross section, Poisson statistics, solid state nuclear track detectors
- MeSH terms
- Alpha Particles, Animals, Cell Line, Cell Nucleus, Cell Survival, Cricetulus, Cytosol, Fibroblasts, Cricetinae, Image Processing, Computer-Assisted, Mathematical Computing, Microscopy, Fluorescence, Monte Carlo Method, Linear Energy Transfer
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