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Improved Method for the Determination of Antimicrobial Activity of Essential Oils in Agar Medium
Abstract
The influence of the number of bacterial cells inoculated and the emulsifying agent used to disperse essential oils (EO) into the culture media on the measurement of the antibacterial activity of EO in an agar medium was determined. The results showed that EO (oregano, thyme and clove) were most effective as antimicrobial agents when the bacterial load was low. Minimum inhibitory concentrations (MICs) were found to vary as a function of the emulsifying agent used. However, MICs obtained by dispersing EOs into 0.2% agar solution without the use of solvents and detergents were greatly reduced compared to when they were used. This demonstrates the fact that solvents and detergents often used in antimicrobial studies significantly decrease the antibacterial activity of EO.
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Citations
Cited by
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488
Antimicrobial activity of essential oils and other plant extracts
Hammer, K. A., Carson, C. F., & Riley, T. V. (1999) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1999.00780.x · Cited by 2,560
Lambert, R. J. W., Skandamis, P. N., Coote, P. J., & Nychas, G.-J. E. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01428.x · Cited by 2,449
Application of natural antimicrobials for food preservation
Tiwari, B. K., Valdramidis, V. P., O’ Donnell, C. P., Muthukumarappan, K., Bourke, P., & Cullen, P. J. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf900668n · Cited by 851
Methods to study the phytochemistry and bioactivity of essential oils
Lahlou, M. (2004) · Phytotherapy Research · doi:10.1002/ptr.1465 · Cited by 398
Metadata
- Authors
- Remmal, A., Bouchikhi, T., Rhayour, K., Ettayebi, M., & Tantaoui-Elaraki, A.
- Venue
- Journal of Essential Oil Research
- Publisher
- Informa UK Limited
- Published
- 1993-03
- Volume
- 5
- Issue
- 2
- Pages
- 179-184
- ISSN
- 1041-2905 (ISSN-L)
- 2163-8152
- OpenAlex
- W1601372800
- Cited by
- 249
- References
- 0
- FWCI
- 2.07
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Improved Method for the Determination of Antimicrobial Activity of Essential Oils in Agar Medium. https://app.cochat.ai/papers/10.1080/10412905.1993.9698197
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- Remmal, A., Bouchikhi, T., Rhayour, K., Ettayebi, M., & Tantaoui-Elaraki, A. (1993). Improved Method for the Determination of Antimicrobial Activity of Essential Oils in Agar Medium. Journal of Essential Oil Research. https://doi.org/10.1080/10412905.1993.9698197
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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