A comprehensive screening shows that ergothioneine is the most abundant antioxidant in the wild macrofungus Phylloporia ribis Ryvarden
Abstract
The polar and non-polar extracts from the authenticated wild mushroom Phylloporia ribis were separated by hydrophilic interaction liquid chromatography (HILIC) and by reverse phase (RP)-HPLC, respectively. A split valve separated the eluents into two fractions for free-radical scavenging analysis and for structural identification. Forty-six compounds showed scavenging activity of the stable-free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The structures of 8 antioxidants (inosine, caffeic acid, ergothioneine, p-hydroxybenzoic acid, adenosine, 3,4-dihydroxybenzaldehyde, apigenin, and naringenin) are characterized by Mass Spectrometer. Among them, ergothioneine was the most abundant (>65%) and most active antioxidant in P. ribis.
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In mushrooms, ERG is typically the main antioxidant, and it may also inhibit tyrosinase.
“This also seems true of mushrooms(271,272), where ERG is typically the main antioxidant(273–275), and where it may also inhibit the oxidative enzyme tyrosinase(276).”
From The biology of ergothioneine, an antioxidant nutraceutical (2020) · cites this paper as [274]
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The biology of ergothioneine, an antioxidant nutraceutical
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- Authors
- Zhao, H., Zhang, M., Liu, Q., Wang, X., Zhao, R., Geng, Y., Wong, T., Li, S., & Wang, X.
- Affiliations
- Qilu University of Technology (CN)
- Biological Sciences, University of Memphis, Memphis, Tennessee, USA
- Linyi City Chinese Medicine Hospital (CN)
- Corresponding author
- Zhao, Hengqiang
- Wang, Xiao
- Venue
- Journal of Environmental Science and Health, Part C
- Publisher
- Informa UK Limited
- Published
- 2018-04-03
- Volume
- 36
- Issue
- 2
- Pages
- 98-111
- ISSN
- 1059-0501 (ISSN-L)
- 1532-4095
- OpenAlex
- W2800129091
- Cited by
- 14
- References
- 50
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 2 · 2019: 1
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Environmental Science and Health Part C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- China Agriculture Research System (CARS-21)
- The Natural Science Foundation of Shandong Province (ZR2017LH071, ZR2016YL006, ZR2013HL029)
- Key Project of Shandong Province (2016GSF202014)
- The Shandong Province Taishan Scholar Program (Lanping Guo)
- The Priority Research Program of the Shandong Academy of Sciences (Lanping Guo)
- Topics
- Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Keywords
- ergothioneine, Phylloporia ribis, wild mushrooms, antioxidants, free radical scavenging, DPPH assay, mushroom extracts, caffeic acid, apigenin, naringenin, inosine, adenosine, p-hydroxybenzoic acid, 3,4-dihydroxybenzaldehyde, natural product screening
- MeSH terms
- Antioxidants, Basidiomycota, Chromatography, Liquid, Ergothioneine, Mass Spectrometry, Free Radical Scavengers, Chromatography, Reverse-Phase
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