Cited by 23Photodynamic Therapy Research StudiesNanoplatforms for cancer theranosticsPorphyrin and Phthalocyanine Chemistry

Induction of oxidative cell damage by photo-treatment with zinc metaN-methylpyridylporphyrin

Al-Mutairi, Dalal A., Al-Mutairi, Dalal A., Craik, James D., Batinic-Haberle, Ines, Benov, Ludmil T.·2007·Free Radical Research

Abstract

We have previously reported that isomeric Zn(II) N-methylpyridylporphyrins (ZnTM-2(3,4)-PyP4 + ) can act as photosensitizers with efficacy comparable to that of hematoporphyrin derivative (HpD) in preventing cell proliferation and causing cell death in vitro. To better understand the biochemical basis of this activity, the effects of photo-activated ZnTM-3-PyP4 + on GSH/GSSG ratio, lipid peroxidation, membrane permeability, oxidative DNA damage, and the activities of SOD, catalase, glutathione reductase, and glutathione peroxidase were evaluated. Light exposure of ZnTM-3-PyP4 + -treated colon adenocarcinoma cells caused a wide spectrum of oxidative damage including depletion of GSH, inactivation of glutathione reductase and glutathione peroxidase, oxidative DNA damage and peroxidation of membrane lipids. Cell staining with Hoechst-33342 showed morphological changes consistent with both necrotic and apoptotic death sequences, depending upon the presence of oxygen.

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Crossref · 10.1080/10715760600952869

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  • Fluorescent light-excited zinc meta N-methylpyridylporphyrin induces oxidative DNA damage in human colon carcinoma LS174T cells, tentatively assigned as 8-oxoGua.

    “Fluorescent light-excited zinc meta N-methylpyridylporphyrin has been shown to induce oxidatively generated damage to DNA of human colon carcinoma LS174T cells, tentatively assigned as 8-oxoGua (184) on the basis of somewhat questionable immunodetection (185).”

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Authors
Al-Mutairi, D. A., Al-Mutairi, D. A., Craik, J. D., Batinic-Haberle, I., & Benov, L. T.
Affiliations
Kuwait University (KW)
Corresponding author
Benov, Ludmil T.
Venue
Free Radical Research
Published
2007-01
Volume
41
Issue
1
Pages
89-96
ISSN
  • 1029-2470 (ISSN-L)
  • 1071-5762
OpenAlex
W2065734399
Cited by
23
References
33
Citations by year
2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
0.89
Type
journal article
Language
en
Open access
closed
Locations
  • Free Radical Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (U19 AI67798-01, U19AI067798)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
  • Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
Keywords
photosensitizers, oxidative cell damage, hematoporphyrin derivative, colon adenocarcinoma cells, glutathione depletion, lipid peroxidation, oxidative DNA damage, glutathione reductase, glutathione peroxidase, superoxide dismutase, catalase, apoptosis, necrosis, photodynamic therapy
MeSH terms
Catalase, Cell Membrane, DNA Damage, Glutathione, Glutathione Peroxidase, Glutathione Reductase, Humans, Metalloporphyrins, Photosensitivity Disorders, Superoxide Dismutase, Lipid Peroxidation, Photosensitizing Agents, Oxidative Stress, Glutathione Disulfide, Cell Line, Tumor

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