Assessment of anti-biofilm activity and bifidogenic growth stimulator (BGS) effect of lyophilized exopolysaccharides (l-EPSs) from Lactobacilli strains
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In the present study, a total of used six lactic acid bacterial strains that isolated from children’s feces and milk products. This study investigated the exopolysacch aride production of these strains. In the culture broth, highest exopolysaccharide production capacity was detected in GD-11, LB-69, and B-3 strains. Monosaccharide compositions of the exopolysaccharides that were purified and lyophilized in the culture medium were investigated and dominant monomer was found to be mannose in all strains. The biofilm-forming capacity of 13 pathogen bacteria was determined and four strains with the highest biofilm-forming capacity were selected as test bacteria for anti-biofilm studies. Different concentrations of l-exopolysaccharides of three lactic acid bacteria were tested for anti-biofilm effect. Highest anti-biofilm effects were exhibited by the L. fermentum LB-69 (90%) against B. cereus RSKK 863. In the statistical analyses performed using the available data, a strong correlation was found between the amount of mannose present in l-exopolysaccharides and the anti-biofilm activity exhibited by l-exopolysaccharides (p < 0.01). In this study, it was concluded that the l-exopolysaccharides of the L. fermentum LB-69 strain could be used as efficient agents with both bifidogenic growth stimulator effect and anti-biofilm effect. If these agents are taken via oral route, it would be possible to provide beneficial effects for the host by increasing the development of bifidobacteria present in the gastrointestinal tract as well as to prevent and treat the diseases by hindering the biofilm formation by harmful bacteria.
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Antibiofilm activity of EPS from different LAB has been reported against pathogenic bacteria such as E. faecalis, B. cereus and P. aeruginosa.
“Antibiofilm activity of EPS from different LAB has been reported against pathogenic bacteria such as E. faecalis, B. cereus and P. aeruginosa [149].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [149]
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- Authors
- Sarikaya, H., Aslim, B., & Yuksekdag, Z.
- Affiliations
- Gazi University (TR)
- Gazi University, Faculty of Science, Department of Biology, Ankara, Turkey
- Venue
- International Journal of Food Properties
- Publisher
- Informa UK Limited
- Published
- 2016-09-20
- Volume
- 20
- Issue
- 2
- Pages
- 362-371
- ISSN
- 1094-2912 (ISSN-L)
- 1532-2386
- OpenAlex
- W2327146875
- Cited by
- 65
- References
- 46
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 9 · 2023: 11 · 2022: 7 · 2021: 8 · 2020: 9 · 2019: 8 · 2018: 1 · 2016: 1
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Food Properties (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- exopolysaccharides, antibiofilm activity, Lactobacillus fermentum, lactobacilli, biofilm formation, Bacillus cereus, mannose, monosaccharide composition, Bifidobacterium, lactic acid bacteria
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