Cited by 65Probiotics and Fermented FoodsPolysaccharides Composition and ApplicationsBacterial biofilms and quorum sensing

Assessment of anti-biofilm activity and bifidogenic growth stimulator (BGS) effect of lyophilized exopolysaccharides (l-EPSs) from Lactobacilli strains

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Sarikaya, Halime, Aslim, Belma, Yuksekdag, Zehranur·2017·International Journal of Food Properties

Abstract

In the present study, a total of used six lactic acid bacterial strains that isolated from children’s feces and milk products. This study investigated the exopolysacch aride production of these strains. In the culture broth, highest exopolysaccharide production capacity was detected in GD-11, LB-69, and B-3 strains. Monosaccharide compositions of the exopolysaccharides that were purified and lyophilized in the culture medium were investigated and dominant monomer was found to be mannose in all strains. The biofilm-forming capacity of 13 pathogen bacteria was determined and four strains with the highest biofilm-forming capacity were selected as test bacteria for anti-biofilm studies. Different concentrations of l-exopolysaccharides of three lactic acid bacteria were tested for anti-biofilm effect. Highest anti-biofilm effects were exhibited by the L. fermentum LB-69 (90%) against B. cereus RSKK 863. In the statistical analyses performed using the available data, a strong correlation was found between the amount of mannose present in l-exopolysaccharides and the anti-biofilm activity exhibited by l-exopolysaccharides (p < 0.01). In this study, it was concluded that the l-exopolysaccharides of the L. fermentum LB-69 strain could be used as efficient agents with both bifidogenic growth stimulator effect and anti-biofilm effect. If these agents are taken via oral route, it would be possible to provide beneficial effects for the host by increasing the development of bifidobacteria present in the gastrointestinal tract as well as to prevent and treat the diseases by hindering the biofilm formation by harmful bacteria.

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Crossref · 10.1080/10942912.2016.1160923

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  1. Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Roberts, P.A.; Smith, F. Colorimetric Determination of Sugars and Related Substances. Analytical Chemistry 1956, 28, 350-356.
  2. Marshall, V.M.; Courie, E.N.; Moreton, R.S. Analysis and Production of Two Exopolysaccharides from Lactobacillus Lactis Subsp. Cremoris LC330. Journal of Dairy Research 1995, 62, 621-628.

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Authors
Sarikaya, H., Aslim, B., & Yuksekdag, Z.
Affiliations
  • Gazi University (TR)
  • Gazi University, Faculty of Science, Department of Biology, Ankara, Turkey
Venue
International Journal of Food Properties
Published
2016-09-20
Volume
20
Issue
2
Pages
362-371
ISSN
  • 1094-2912 (ISSN-L)
  • 1532-2386
OpenAlex
W2327146875
Cited by
65
References
46
Citations by year
2026: 2 · 2025: 9 · 2024: 9 · 2023: 11 · 2022: 7 · 2021: 8 · 2020: 9 · 2019: 8 · 2018: 1 · 2016: 1
FWCI
4.20
Type
journal article
Language
en
Open access
Locations
International Journal of Food Properties (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
exopolysaccharides, antibiofilm activity, Lactobacillus fermentum, lactobacilli, biofilm formation, Bacillus cereus, mannose, monosaccharide composition, Bifidobacterium, lactic acid bacteria

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