The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence
Abstract
The serum amyloid A protein is one of the major reactants in the acute-phase response. Using representational difference analysis comparing RNA from normal and involuting quarters of a dairy cow mammary gland, we found an mRNA encoding the SAA3 protein (M-SAA3). The M-SAA3 mRNA was localized to restricted populations of bovine mammary epithelial cells (MECs). It was expressed at a moderate level in late pregnancy, at a low level through lactation, was induced early in milk stasis, and expressed at high levels in most MECs during mid to late involution and inflammation/mastitis. The mature M-SAA3 peptide was expressed in Escherichia coli, antibodies made, and shown to have antibacterial activity against E. coli, Streptococcus uberis and Pseudomonas aeruginosa. These results suggest that the mammary SAA3 may have a role in protection of the mammary gland during remodelling and infection and possibly in the neonate gastrointestinal tract.
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Transformed Chlamydomonas reindhartii chloroplast accumulated 5% total soluble protein of the mammary-associated serum amyloid A3 peptide, but its antimicrobial activity was not tested.
“Transformed Chlamydomonas reindhartii chloroplast accumulated 5% total soluble protein (TSP) of the mammary-associated serum amyloid A3 peptide, but its antimicrobial activity was not tested [80,81].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [81]
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- Authors
- Molenaar, A. J., Harris, D. P., Rajan, G. H., Pearson, M. L., Callaghan, M. R., Sommer, L., Farr, V. C., Oden, K. E., Miles, M. C., Petrova, R. S., Good, L. L., Singh, K., McLaren, R. D., Prosser, C. G., Kim, K. S., Wieliczko, R. J., Dines, M. H., Johannessen, K. M., Grigor, M. R., Davis, S. R., & Stelwagen, K.
- Affiliations
- AgResearch (NZ)
- Corresponding author
- Molenaar, Adrian J.
- Venue
- Biomarkers
- Publisher
- Informa UK Limited
- Published
- 2009-02
- Volume
- 14
- Issue
- 1
- Pages
- 26-37
- ISSN
- 1354-750X (ISSN-L)
- 1366-5804
- OpenAlex
- W1989155338
- Cited by
- 93
- References
- 66
- Citations by year
- 2025: 5 · 2024: 1 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 8 · 2013: 5 · 2012: 9
- FWCI
- 23.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomarkers (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Milk Quality and Mastitis in Dairy Cows
- Health Sciences › Veterinary › Small Animals › Animal health and immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- serum amyloid A3, acute phase response, bovine mammary gland, mammary epithelial cells, mammary gland involution, mastitis, milk stasis, antibacterial activity, host defense, dairy cattle, Streptococcus uberis, Pseudomonas aeruginosa, Escherichia coli
- MeSH terms
- Serum Amyloid A Protein, Animals, Base Sequence, Cattle, Enzyme-Linked Immunosorbent Assay, Mammary Glands, Animal, RNA, Messenger, Blotting, Northern, Blotting, Western, Polymerase Chain Reaction, DNA Primers
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