Pneumocystisjirovecii dihydropteroate synthase genotypes in French patients with pneumocystosis: a 1998–2001 prospective study
Abstract
Dihydropteroate synthase gene (DHPS) mutations at codons 55 and 57 have been associated with sulfa/sulfone resistance in Pneumocystis jirovecii strains from patients who previously received prophylaxis. To evaluate the prevalence of these mutations, a portion of P. jirovecii DHPS gene was analysed using PCR combined with restriction fragment length polymorphism (RFLP) analysis in 92 bronchoalveolar fluid samples collected between January 1998 and September 2001 from French patients with pulmonary pneumocystosis (PCP). Seventy-six samples contained the wild-type DHPS genotype (82.6%) and 16 contained a mutant genotype (17.4%). Twelve out of the 16 isolates with a mutant DHPS genotype corresponded to patients who had never received sulfa or sulfone prophylaxis, suggesting that DHPS mutants may be acquired de novo. There was no significant difference in favourable or adverse outcome in PCP caused by the wild or mutant DHPS genotypes (P = 0.34).
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The presence of DHPS mutations in patients without prior TMP-SMX or dapsone use for PCP prophylaxis, the absence of similar mutations in Pneumocystis isolated from other mammalian species, and the impact of geography on DHPS genotype have substantial implications for disease transmission (i.e., person-to-person transmission).
“The presence of DHPS mutations in patients without prior TMP-SMX or dapsone use for PCP prophylaxis, the absence of similar mutations in Pneumocystis isolated from other mammalian species, and the impact of geography on DHPS genotype have substantial implications for disease transmission (i.e., person-to-person transmission) that are beyond the scope of this review (32–35).”
From Dihydropteroate Synthase Gene Mutations in<i>Pneumocystis</i>and Sulfa Resistance (2004) · cites this paper as [34]
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Dihydropteroate Synthase Gene Mutations in<i>Pneumocystis</i>and Sulfa Resistance
Huang, L., Crothers, K., Atzori, C., Benfield, T., Miller, R., Rabodonirina, M., & Helweg-Larsen, J. (2004) · Emerging Infectious Diseases · doi:10.3201/eid1010.030994 · Cited by 131 · see citing works
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- Authors
- Latouche, S., Lacube, P., Maury, E., Bolognini, J., Develoux, M., Girard, P., Godet, C., Lebrette, M., Mayaud, C., Guillot, J., & Roux, P.
- Affiliations
- Assistance Publique – Hôpitaux de Paris (FR)
- Bipar (FR)
- Corresponding author
- Latouche, S.
- Venue
- Medical Mycology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-01
- Volume
- 41
- Issue
- 6
- Pages
- 533-537
- ISSN
- 0268-1218 (ISSN-L)
- 1369-3786
- 1460-2709
- OpenAlex
- W2090042039
- Cited by
- 19
- References
- 12
- Citations by year
- 2022: 2 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medical Mycology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Pneumocystis jirovecii pneumonia detection and treatment
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Pneumocystis jirovecii, dihydropteroate synthase, dhps mutations, pneumocystosis, drug resistance, prophylaxis, de novo mutations, bronchoalveolar lavage, clinical outcomes
- MeSH terms
- Dihydropteroate Synthase, DNA, Fungal, France, Genotype, Humans, Mutation, Pneumocystis, Pneumonia, Pneumocystis, Prospective Studies, Polymorphism, Restriction Fragment Length
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