Cited by 27Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesFungal Plant Pathogen Control

Phenotypic and genotypic evaluation of fluconazole resistance in vaginal Candida strains isolated from HIV-infected women from Brazil

Ribeiro, Mariceli A., Paula, C. R., Perfect, John R., Cox, Gary M.·2005·Medical Mycology

Abstract

We conducted a study to determine the antifungal susceptibility of vaginal Candida isolates from HIV-infected Brazilian women. Among 127 women enrolled, positive cultures for yeast were obtained from 31 of 38 (81%) women with symptomatic vulvovaginitis, and from 41 of 89 (46%) asymptomatic women. Susceptibility testing demonstrated 11 of the 72 isolates had either resistance or dose-dependent susceptibility to azole drugs, including four Candida albicans strains. Expression of the MDR1, CDR1, CDR2 and ERG11 genes was evaluated in all of the C. albicans isolates, and all four of the strains with reduced susceptibility to fluconazole had increased expression of CDR1 as compared to the fluconazole-sensitive strains. No increased expression of the other genes was identified. This large survey of Candida isolates from HIV-infected women from Brazil demonstrates that reduced susceptibility to azoles occurs at a low frequency among vaginal yeast isolates, and when present in C. albicans, azole resistance is associated with increased expression of CDR1.

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Crossref · 10.1080/13693780500093838

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16 of 16 references verified0 citation errors · 0 not checkable

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Authors
Ribeiro, M. A., Paula, C. R., Perfect, J. R., & Cox, G. M.
Affiliations
  • Universidade Federal do Espírito Santo (BR)
  • Universidade de São Paulo (BR)
  • Duke University (US)
Venue
Medical Mycology
Published
2005-01
Volume
43
Issue
7
Pages
647-650
ISSN
  • 0268-1218 (ISSN-L)
  • 1369-3786
  • 1460-2709
OpenAlex
W2029416365
Cited by
27
References
16
Citations by year
2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
FWCI
1.49
Type
journal article
Language
en
Open access
closed
Locations
  • Medical Mycology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
Keywords
fluconazole resistance, Candida albicans, vaginal candidiasis, women living with HIV, azole resistance, MDR1, CDR2, ERG11, antifungal susceptibility, vulvovaginitis, Brazilian women
MeSH terms
Adult, Antifungal Agents, Brazil, Candida, Candidiasis, Vulvovaginal, DNA, Fungal, Evaluation Studies as Topic, Female, Fungal Proteins, Genotype, Humans, Microbial Sensitivity Tests, HIV Infections, Fluconazole, AIDS-Related Opportunistic Infections, Drug Resistance, Fungal, Membrane Transport Proteins

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