Cited by 55Metabolomics and Mass Spectrometry StudiesCancer and biochemical researchAdvanced Chemical Sensor Technologies

Toxicometabolomics of Urinary Biomarkers for Human Gastric Cancer in a Mouse Model

Kim, Kyu-Bong, Yang, Ji-Young, Kwack, Seung Jun, Park, Kui Lea, Kim, Hyung Sik, Ryu, Do Hyun, Kim, Yeon-Joo, Hwang, Geum-Sook +1 more·2010·Journal of Toxicology and Environmental Health, Part A

Abstract

Toxicometabolomics of urinary biomarkers for human gastric cancer in a mouse model was investigated using (1)H-nuclear magnetic resonance (NMR) spectroscopy. A human gastric adenocarcinoma cell line (1 × 10(7) cells/ml) was grafted onto the skin of the back of intact male BALB/c-nu/nu mice. After the xenografted tumors developed, urine was collected and analyzed for endogenous metabolites. Global profiling combined with principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal projections to latent squares-discriminant analysis (OPLS-DA) showed distinct separation of clusters between control and tumor-bearing mice. Targeted profiling revealed significant changes in trimethylamine oxide (TMAO), 3-indoxylsulfate, hippurate, and citrate levels in mice carrying human gastric cancer cells compared to normal mice. The levels of TMAO (0.41-fold) and hippurate (0.26-fold) in tumor-bearing mice were significantly decreased, whereas the levels of 3-indoxylsulfate (3.39-fold), 2-oxoglutarate (2.32-fold), and citrate (1.9-fold) were significantly increased in urine samples of tumor-bearing mice. Data suggest that TMAO, hippurate, 3-indoxylsulfate, 2-oxoglutarate, and citrate may serve as useful urinary biomarkers for gastric tumorigenesis in a mouse model.

Integrity signals

Registry

Crossref · 10.1080/15287394.2010.511545

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Kim, K.-B., Yang, J.-Y., Kwack, S. J., Park, K. L., Kim, H. S., Ryu, D. H., Kim, Y.-J., Hwang, G.-S., & Lee, B. M.
Affiliations
  • Inje University (KR)
  • National Institute of Food and Drug Safety Evaluation (KR)
  • Pusan National University (KR)
  • Sungkyunkwan University (KR)
  • Chungnam National University (KR)
Corresponding author
  • Hwang, Geum-Sook
  • Lee, Byung Mu
Venue
Journal of Toxicology and Environmental Health, Part A
Published
2010-11
Volume
73
Issue
21-22
Pages
1420-1430
ISSN
  • 0098-4108 (ISSN-L)
  • 1528-7394
  • 1087-2620
OpenAlex
W2146796971
Cited by
55
References
48
Citations by year
2024: 3 · 2023: 1 · 2022: 2 · 2021: 10 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 4 · 2012: 5
FWCI
1.56
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Toxicology and Environmental Health (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
  • Physical Sciences › Engineering › Biomedical Engineering › Advanced Chemical Sensor Technologies
Keywords
gastric cancer, urinary biomarkers, xenograft mouse model, trimethylamine N-oxide, hippurate, citrate, alpha-ketoglutarate, endogenous metabolites, gastric carcinogenesis, NMR metabolomics
MeSH terms
Adenocarcinoma, Animals, Disease Models, Animal, Hippurates, Humans, Indican, Ketoglutaric Acids, Male, Methylamines, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, Magnetic Resonance Spectroscopy, Stomach Neoplasms, Biomarkers, Tumor, Discriminant Analysis, Citric Acid, Xenograft Model Antitumor Assays, Principal Component Analysis, Cell Line, Tumor, Mice, Metabolomics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review