Toxicometabolomics of Urinary Biomarkers for Human Gastric Cancer in a Mouse Model
Abstract
Toxicometabolomics of urinary biomarkers for human gastric cancer in a mouse model was investigated using (1)H-nuclear magnetic resonance (NMR) spectroscopy. A human gastric adenocarcinoma cell line (1 × 10(7) cells/ml) was grafted onto the skin of the back of intact male BALB/c-nu/nu mice. After the xenografted tumors developed, urine was collected and analyzed for endogenous metabolites. Global profiling combined with principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal projections to latent squares-discriminant analysis (OPLS-DA) showed distinct separation of clusters between control and tumor-bearing mice. Targeted profiling revealed significant changes in trimethylamine oxide (TMAO), 3-indoxylsulfate, hippurate, and citrate levels in mice carrying human gastric cancer cells compared to normal mice. The levels of TMAO (0.41-fold) and hippurate (0.26-fold) in tumor-bearing mice were significantly decreased, whereas the levels of 3-indoxylsulfate (3.39-fold), 2-oxoglutarate (2.32-fold), and citrate (1.9-fold) were significantly increased in urine samples of tumor-bearing mice. Data suggest that TMAO, hippurate, 3-indoxylsulfate, 2-oxoglutarate, and citrate may serve as useful urinary biomarkers for gastric tumorigenesis in a mouse model.
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TMAO was used as a urinary marker in a mouse model of gastric tumorigenesis.
“TMAO was used also as a urinary marker in a mouse model of gastric tumorigenesis (60).”
From Trimethylamine <i>N</i> -Oxide, the Microbiome, and Heart and Kidney Disease (2017) · cites this paper as [60]
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Trimethylamine <i>N</i> -Oxide, the Microbiome, and Heart and Kidney Disease
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- Authors
- Kim, K.-B., Yang, J.-Y., Kwack, S. J., Park, K. L., Kim, H. S., Ryu, D. H., Kim, Y.-J., Hwang, G.-S., & Lee, B. M.
- Affiliations
- Inje University (KR)
- National Institute of Food and Drug Safety Evaluation (KR)
- Pusan National University (KR)
- Sungkyunkwan University (KR)
- Chungnam National University (KR)
- Corresponding author
- Hwang, Geum-Sook
- Lee, Byung Mu
- Venue
- Journal of Toxicology and Environmental Health, Part A
- Publisher
- Informa UK Limited
- Published
- 2010-11
- Volume
- 73
- Issue
- 21-22
- Pages
- 1420-1430
- ISSN
- 0098-4108 (ISSN-L)
- 1528-7394
- 1087-2620
- OpenAlex
- W2146796971
- Cited by
- 55
- References
- 48
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 2 · 2021: 10 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 3 · 2013: 4 · 2012: 5
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Toxicology and Environmental Health (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Chemical Sensor Technologies
- Keywords
- gastric cancer, urinary biomarkers, xenograft mouse model, trimethylamine N-oxide, hippurate, citrate, alpha-ketoglutarate, endogenous metabolites, gastric carcinogenesis, NMR metabolomics
- MeSH terms
- Adenocarcinoma, Animals, Disease Models, Animal, Hippurates, Humans, Indican, Ketoglutaric Acids, Male, Methylamines, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, Magnetic Resonance Spectroscopy, Stomach Neoplasms, Biomarkers, Tumor, Discriminant Analysis, Citric Acid, Xenograft Model Antitumor Assays, Principal Component Analysis, Cell Line, Tumor, Mice, Metabolomics
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