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Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer
Open accessAbstract
Pancreatic cancer presents with a dismal mortality rate and is in urgent need of methods for early detection with potential for timely intervention. All living cells, including cancer cells, generate exosomes. We previously discovered double stranded genomic DNA in exosomes derived from the circulation of pancreatic cancer patients, which enabled the detection of prevalent mutations associated with the disease. Here, we report a proof-of-concept study that demonstrates the potential clinical utility of circulating exosomal DNA for identification of KRASG12D and TP53R273H mutations in patients with pancreas-associated pathologies, including pancreatic ductal adenocarcinoma (PDAC), chronic pancreatitis (CP) and intraductal papillary mucinous neoplasm (IPMN), and in healthy human subjects. In 48 clinically annotated serum samples from PDAC patients, digital PCR analyses of exosomal DNA identified KRASG12D mutation in 39.6% of cases, and TP53R273H mutation in 4.2% of cases. KRASG12D and TP53R273H mutations were also detected in exosomal DNA from IPMN patients (2 out of 7 with KRASG12D, one of which also co-presented with TP53R273H mutation). Circulating exosomal DNA in 5 out of 9 CP patients enabled the detection of KRASG12D mutation. In 114 healthy subject-derived circulating exosomal DNA, 2.6% presented with KRASG12D mutation and none with TP53R273H mutation. This study highlights the value of circulating exosomal DNA for a rapid, low-cost identification of cancer driving mutations. The identification of mutations in IPMN patients and healthy subjects suggests that liquid biopsies may allow potential assessment of cancer risk but with a cautionary note that detection of clinical cancer cannot be assumed.
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“In pancreatic cancer, liquid biopsy using circulating cfDNA proves valuable for diagnosis and monitoring.”
From Significance of TP53, CDKN2A, SMAD4 and KRAS in Pancreatic Cancer (2024) · cites this paper as [30]
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“A study successfully detected KRASG12D and TP53R273H mutations in exosomal DNA from PDAC patients, showcasing the potential role of liquid biopsy [30].”
From Significance of TP53, CDKN2A, SMAD4 and KRAS in Pancreatic Cancer (2024) · cites this paper as [30]
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Cites
SEER Cancer Statistics Review, 1975-2003
Ries, L. A. G., Harkins, D., Krapcho, M. F., Mariotto, A. B., Miller, B., Feuer, E. J., Clegg, L. X., Eisner, M. P., Horner, M., Howlader, N., et al. (2006) · Digital Archive @ GSU · Cited by 11,404
Cell-free nucleic acids as biomarkers in cancer patients
Schwarzenbach, H., Hoon, D. S. �., & Pantel, K. (2011) · Nature reviews. Cancer · doi:10.1038/nrc3066 · Cited by 2,897
Drugging the undruggable RAS: Mission Possible?
Cox, A. D., Fesik, S. W., Kimmelman, A. C., Luo, J., & Der, C. J. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4389 · Cited by 1,997
The biology and function of exosomes in cancer
Kalluri, R. (2016) · Journal of Clinical Investigation · doi:10.1172/jci81135 · Cited by 1,880
Self-citationBiogenesis and secretion of exosomes
Kowal, J., Tkach, M., & Théry, C. (2014) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2014.05.004 · Cited by 1,781
Double-stranded DNA in exosomes: a novel biomarker in cancer detection
Thakur, B. K., Zhang, H., Becker, A., Matei, I. R., Huang, Y., Costa‐Silva, B., Zheng, Y., Hoshino, A., Brazier, H., Xiang, J., et al. (2014) · Cell Research · doi:10.1038/cr.2014.44 · Cited by 1,629
Genetics and biology of pancreatic ductal adenocarcinomaPreprint
Hezel, A. F., Kimmelman, A. C., Stanger, B. Z., Bardeesy, N. M., & DePinho, R. A. (2006) · Genes & Development · doi:10.1101/gad.1415606 · Cited by 1,611
Kahlert, C., Melo, S. A., Protopopov, A. I., Tang, J., Seth, S., Koch, M., Zhang, J., Weitz, J., Chin, L., Futreal, P. A., et al. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.c113.532267 · Cited by 1,058
Self-citationExosomes in tumor microenvironment influence cancer progression and metastasis
Kahlert, C., & Kalluri, R. (2013) · Journal of Molecular Medicine · doi:10.1007/s00109-013-1020-6 · Cited by 888
Self-citationHuggett, J. F., Foy, C. A., Benes, V., Emslie, K., Garson, J. A., Haynes, R., Hellemans, J., Kubista, M., Mueller, R. D., Nolan, T., et al. (2013) · Clinical Chemistry · doi:10.1373/clinchem.2013.206375 · Cited by 859
Research works printed but not linked: 2
- Popovic Hadzija M, 2007, Croat Med J, 48, 218
- 10.1200/jco/1999.17.2.578 · DOI
Cited by
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Items 201–248 of 248
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Iafolla, M. A. J., Picardo, S. L., Aung, K. L., & Hansen, A. R. (2020) · JNCI Cancer Spectrum · doi:10.1093/jncics/pkaa050 · Cited by 3
The underexplored complexity of pancreatic cancer: Early dissemination and quiescence
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Velmiskina, A. A., Kalinina, O., Petrova, T. A., Nikitin, Y. V., & Головкин, А. С. (2022) · Russian Journal for Personalized Medicine · doi:10.18705/2782-3806-2022-2-3-101-110 · Cited by 2
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Löhr, M. J., Kordes, M., Rutkowski, W., Heuchel, R. L., Gustafsson‐Liljefors, M., Russom, A., & Nilsson, M. F. (2018) · Expert Review of Precision Medicine and Drug Development · doi:10.1080/23808993.2018.1476061 · Cited by 2
Primary and Secondary Prevention of Pancreatic Cancer
Yu, A., Romero, T., & Genkinger, J. M. (2019) · Current Epidemiology Reports · doi:10.1007/s40471-019-00189-2 · Cited by 2
Role of Horizontal Gene Transfer in Cancer Progression
Abril, A. G., & Notario, V. (2019) · Springer eBooks · doi:10.1007/978-3-030-21862-1_17 · Cited by 3
Garcı́a-Silva, S., Ximénez‐Embún, P., Muñoz, J., & Peinado, H. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1205-7_25 · Cited by 2
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Farmer, M., Redd, K., Roberson, T., Smith, M., & Lewis, K. A. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-821789-4.00021-7 · Cited by 1
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Comprehensive molecular-clinical profiling of cholangiocarcinoma according to pathologic subtypes
Ahn, K. S., O'Brien, D., Kang, Y. N., Mounajjed, T., Lee, H. W., Kim, Y. H., Kim, T., Kim, M. W., Han, J. Y., Borad, M. J., et al. (2026) · HPB · doi:10.1016/j.hpb.2026.02.007 · Cited by 1
Wu, T., Dickinson, K., Schiller, I., Tsering, T., Chen, Y.-I., Nadeau, A., Dendukuri, N., Agnihotram, V. R., & Burnier, J. V. (2026) · Journal of Extracellular Biology · doi:10.1002/jex2.70123 · Cited by 1
Stone, T. W., Clanchy, F. I. L., & Williams, R. O. (2026) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2026.1885761 · Cited by 1
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Scalia, P., Williams, S. J., Russo, A. D., & Giordano, A. (2017) · Current clinical pathology · doi:10.1007/978-3-319-55661-1_8 · Cited by 1
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Korc, M., & McElyea, S. D. (2017) · Springer eBooks · doi:10.1007/978-1-4939-6631-8_67-1 · Cited by 1
Liu, D., Prado, M. M., Giovannetti, E., Jiao, L. R., Krell, J., & Frampton, A. E. (2019) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2019.1622414 · Cited by 1
Zheng, D., Grandgenett, P. M., Zhang, Q., Baine, M. J., Shi, Y., Du, Q., Liang, X., Wong, J., Iqbal, S., Preuss, K., et al. (2023) · medRxiv · doi:10.1101/2023.11.02.23297995 · Cited by 1
Biomarkers of Pancreatic Cancer
Kishikawa, T., Tada, M., Otsuka, M., & Koike, K. (2019) · Springer eBooks · doi:10.1007/978-981-13-7295-7_9
Ben, F. D., Bulfoni, M., Turetta, M., Brisotto, G., Steffan, A., & Curcio, F. (2019) · La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine · doi:10.23736/s1825-859x.19.00004-5
Gleeson, F. C. (2021) · Gastrointestinal Endoscopy · doi:10.1016/j.gie.2020.07.024
Dpcr Opening More Specific Possibilities for Oncological Diagnosis
Orué, A. (2020) · Biomedical Journal of Scientific & Technical Research · doi:10.26717/bjstr.2020.30.005002
Extracellular vesicles in tumor immunotherapy
Li, J., Stephens, E. N., & Zhang, Y. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-85781-9.00009-9 · Cited by 1
Etiology and early detection of pancreatic cancer
Yu, J. (2021) · Figshare
Li, Y., Wu, J., Lei, J., Zhou, F., Yin, X., Hu, D., Mao, Y., Wu, L., & Liao, W. (2020) · Research Square · doi:10.21203/rs.3.rs-72200/v1
Molecular Screening for Urothelial Cancer: How Close We Are?
Michas, A., Michas, B., Tsitsibis, A., & Tsoukalas, N. (2023) · Global Medical Genetics · doi:10.1055/s-0043-1768958
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Unnikrishnan, K., Rames, A. D. U., & Kumar, R. (2024) · Springer eBooks · doi:10.1007/978-981-97-2494-9_10
Sadeghvand, S., Nasiri, H., Sadighi, A., Heris, J. A., Shahabi, P., Shomali, N., Nasab, R. M., Hejazi, S., Bahadori, A., & Akbari, M. (2023) · Multidisciplinary Cancer Investigation · doi:10.61186/mci.7.4.1
Single Small Extracellular Vesicle Analysis Using Plasmonic and Fluorescence Microscopy
Ohannesian, N., Mallick, M. S., Lin, S. H., & Shih, W. (2024) · IEEE BioSensors Conference (BioSensors) · doi:10.1109/biosensors61405.2024.10712671
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Li, S., Liu, Y., & Yang, D. (2025) · Chinese Science Bulletin (Chinese Version) · doi:10.1360/tb-2024-1236
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Ofori, E. K., & Aidoo, N. E. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-33902-8.00002-x
Ahadova, A., Bohaumilitzky, L., Walle, T., Kraske, J. A., Tariverdian, M., Ganserer‐Schmitt, U., Haußer-Siller, I., Fuchs, V., Nelius, N., Erisen, G. M., et al. (2026) · International Journal of Cancer · doi:10.1002/ijc.70387
Cintas, C. (2017) · theses.fr (ABES) · doi:10.70675/d19569d2z4eaez4a9azae8bz1e155d6b15b3
Yildirim, İ., & Avcı, Ç. B. (2026) · Seminars in Oncology · doi:10.1016/j.seminoncol.2026.152497
Intérêt de l'étude des HSP70-exosomes dans le diagnostic et le suivi du cancer
Chanteloup, G. (2020) · theses.fr (ABES) · doi:10.70675/ae1cca2az7e99z41aazbb58z8b3f455c016a
Blood-based biomarkers for traumatic brain injury
Rezzag, A. (2021) · theses.fr (ABES) · doi:10.70675/330bcff7z665fz4924z9a1bz947c6b1f9484
Trioën, C. (2024) · theses.fr (ABES) · doi:10.70675/7c132518z8997z4d01z8084z87c06a661e5a
Li, J., Du, Z., Liu, X., Pan, T., Zheng, Y., & He, Y. (2026) · Discover Oncology · doi:10.1007/s12672-026-05088-9
Bura, F. I., Rodríguez‐Ortiz, L., Pezzopane, R., Valenzuela-Molina, F., Rufián‐Andújar, B., Martínez-López, A., Romero‐Ruíz, A., Vazquez-Borrego, M. C., & Arjona-Sánchez, Á. (2026) · Research Square · doi:10.21203/rs.3.rs-10736351/v1
Strategy for early diagnosis of pancreatic cancer
Sharma, R. K., Singh, A., Sharma, G., Dawra, S., & Rana, S. S. (2026) · Frontiers in Gastroenterology · doi:10.3389/fgstr.2026.1731223
de la Fuente, E. B., Ximénez‐Embún, P., Muñoz, J., Peinado, H., & Garcı́a-Silva, S. (2026) · Methods in molecular biology · doi:10.1007/978-1-0716-5619-8_13
Metadata
- Authors
- Yang, S., Che, S. P. Y., Kurywchak, P., Tavormina, J. L., Gansmo, L. B., Correa de Sampaio, P., Tachezy, M., Bockhorn, M., Gebauer, F., Haltom, A. R., Melo, S. A., LeBleu, V. S., & Kalluri, R.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- Department of Cancer Biology, Metastasis Research Center, University of Texas MD Anderson Cancer Center, Houston, TX, USA
- University Medical Center Hamburg-Eppendorf (DE)
- Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal (I3S)
- Corresponding author
- Kalluri, Raghu
- ORCID
- Venue
- Cancer Biology & Therapy
- Publisher
- Informa UK Limited
- Published
- 2017-01-25
- Volume
- 18
- Issue
- 3
- Pages
- 158-165
- ISSN
- 1538-4047 (ISSN-L)
- 1555-8576
- PMC
- PMC5389423
- OpenAlex
- W2582660163
- Cited by
- 248
- References
- 42
- Citations by year
- 2026: 15 · 2025: 14 · 2024: 24 · 2023: 27 · 2022: 26 · 2021: 37 · 2020: 38 · 2019: 33 · 2018: 20 · 2017: 13
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cancer Biology & Therapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Texas MD Anderson Cancer Center ((CA016672))
- National Cancer Institute (P30CA016672)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Keywords
- circulating exosomes, exosomal DNA, KRAS G12D mutation, pancreatic ductal adenocarcinoma, intraductal papillary mucinous neoplasm, chronic pancreatitis, liquid biopsy, early cancer detection, cancer risk assessment, digital PCR
- MeSH terms
- DNA, DNA, Neoplasm, Humans, Mutation, Pancreatic Neoplasms, Case-Control Studies, Polymerase Chain Reaction, Tumor Suppressor Protein p53, Proto-Oncogene Proteins p21(ras), Cell Line, Tumor, Exosomes
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