Age-associated DNA methylation changes in Xenopus frogs
Open accessPublished version of a preprint
Abstract
Abstract Age-associated changes in DNA methylation have been widely established and characterized in mammals but not yet extended to other species. We present clear evidence that the aquatic vertebrate species Xenopus tropicalis displays clear patterns of age-associated changes in DNA methylation. This observation allows us to leverage the unique resources available for Xenopus to study how DNA methylation relates to other hallmarks of aging. We have generated whole genome bisulfite sequencing (WGBS) profiles in nine samples representing young, mature and old adults and characterize the gene- and chromosome-scale changes. Moreover, we selected a few thousands CpG sites to expand the findings in a larger cohort of individuals through targeted DNA methylation analysis to produce a cost effective epigenetic clock assay.
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Cites
DNA methylation-based measures of biological age: meta-analysis predicting time to death
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- Authors
- Morselli, M., Bennett, R., Shaidani, N.-I., Horb, M., Peshkin, L., & Pellegrini, M.
- Affiliations
- University of California, Los Angeles (US)
- Marine Biological Laboratory (US)
- Corresponding author
- Peshkin, Leonid
- Pellegrini, Matteo
- Venue
- Epigenetics
- Publisher
- Informa UK Limited
- Published
- 2023-04-24
- Volume
- 18
- Issue
- 1
- Pages
- 2201517-2201517
- ISSN
- 1559-2294 (ISSN-L)
- 1559-2308
- PMC
- PMC10128463
- OpenAlex
- W4366822080
- Cited by
- 10
- References
- 9
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 1
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Epigenetics (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Open Access Server of the Woods Hole Scientific Community (Woods Hole Scientific Community) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH (R01 HD073104, P40 OD010997)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Keywords
- DNA methylation, aging, Xenopus tropicalis, epigenetic clocks, targeted bisulfite sequencing, amphibians, skin, whole-genome bisulfite sequencing
- MeSH terms
- Whole Genome Sequencing, Animals, Mammals, Sulfites, Xenopus, Xenopus laevis, Sequence Analysis, DNA, CpG Islands, DNA Methylation
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