Cited by 76Biofield Effects and BiophysicsElectromagnetic Fields and Biological EffectsMicrotubule and mitosis dynamics

Electromagnetic activity of yeast cells in the M phase

Pokorný, J., Hašek, J., Jelínek, F., Šaroch, J., Palán, B.·2001·Electro- and Magnetobiology

Abstract

Electromagnetic activity around yeast mitotic cells (Saccharomyces cerevisiae) was measured in the frequency range 8–9 MHz and special care was taken to extract reliable information from the raw signals. The characteristic of cold-sensitive tubulin mutants tub2-401 and tub2-406, which come to arrest before mitosis at a restrictive temperature (14°C) and which re-enter mitosis upon a shift back to a permissive temperature (28°C), was used to prepare synchronized mitotic cells. Immunofluorescence microscopy using an antitubulin antibody was used to analyze microtubular structures. The arrested tub2-401 mutant that had back-shifted to permissive temperature displayed no microtubules and no electromagnetic activity around the cells. In contrast, the arrested cells of the mutant tub2-406 displayed developed, but aberrant, nonfunctional microtubules and a high electromagnetic activity around the cells. The electromagnetic activity around the arrested mutant tub2-401 back-shifted to permissive temperature peaks at four time points which may coincide with (i) formation of the mitotic spindle, (ii) binding of chromatids to kinetochore microtubules, (iii) elongation of the spindle in anaphase A, and (iv) elongation of the spindle in anaphase B. The profile of the electromagnetic activity around the synchronized mutant tub2-406 at permissive temperature seems to be delayed by the time required for aberrant nonfunctional microtubules to be depolymerized. Experimental results presented in this paper support Pohl's idea of existence of the electromagnetic field around yeast cells.

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Registry

Crossref · 10.1081/jbc-100108577

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28 of 28 references verified0 citation errors · 0 not checkable

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  • Coherent megahertz emissions were detected from living cells and ascribed to microtubule dynamics powered by mitochondrial electromagnetic fields.

    “In 2001 and 2004, coherent megahertz emissions were detected from living cells and ascribed to microtubule dynamics (powered by mitochondrial electromagnetic fields) by the group of Jiri Pokorný in Prague [134,135].”

    From Consciousness in the universe (2014) · cites this paper as [135]

  • Reimers et al. validated 8 MHz coherence measured in microtubules by Pokorný as weak Fröhlich condensation.

    “They validated 8 MHz coherence measured in microtubules by Pokorný [134,135] as weak condensation.”

    From Consciousness in the universe (2014) · cites this paper as [135]

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Authors
Pokorný, J., Hašek, J., Jelínek, F., Šaroch, J., & Palán, B.
Affiliations
Czech Academy of Sciences (CZ)
Venue
Electro- and Magnetobiology
Published
2001-01
Volume
20
Issue
3
Pages
371-396
ISSN
1061-9526 (ISSN-L)
OpenAlex
W2011760920
Cited by
76
References
29
Citations by year
2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 9
FWCI
0.79
Type
journal article
Language
en
Open access
closed
Locations
Electro- and Magnetobiology (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Biofield Effects and Biophysics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
yeast cells, mitosis, Saccharomyces cerevisiae, microtubules, mitotic spindle, anaphase, kinetochore microtubules, cell synchronization, depolymerization

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