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Monocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro.
Open accessAbstract
Human hepatoma cells mimic the acute phase response after treatment with monocyte-conditioned medium. Levels of secreted fibrinogen, alpha-1 acid glycoprotein, C-reactive protein, haptoglobin, and the third component of complement were elevated compared with control levels after 48 h of incubation with conditioned supernatant medium from an enriched fraction of normal peripheral monocytes. Albumin levels declined and alpha-1 antitrypsin remained unchanged. Levels of specific mRNA were measured by hybridization to slot blots and Northern blots and changed in correspondence with protein alterations. Interleukin-1 and tumor necrosis factor stimulated the third component of complement, but did not elevate any other member of the acute phase group and were therefore only partially active in this system. The identification of an in vitro model of the human acute phase response will permit analysis of the molecular basis for coordinate regulation of this group of facultative genes.
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Citations
Cited by
Interleukin-6 and the acute phase response
Heinrich, P. C., Castell, J. V., & Andus, T. (1990) · Biochemical Journal · doi:10.1042/bj2650621 · Cited by 2,734
Gauldie, J., Richards, C., Harnish, D., Lansdorp, P., & Baumann, H. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.20.7251 · Cited by 1,635
Interleukin‐6 is the major regulator of acute phase protein synthesis in adult human hepatocytes
Castell, J. V., Gómez-Lechón, M. J., David, M., Andus, T., Geiger, T., Trullenque, R., Fabra, R., & Heinrich, P. C. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)80476-4 · Cited by 893
Effect of inflammatory cytokines on hypoxia-induced erythropoietin production
Faquin, W., Schneider, T., & Goldberg, M. (1992) · Blood · doi:10.1182/blood.v79.8.1987.1987 · Cited by 532
Devlin, R. B., McDonnell, W. F., Mann, R., Becker, S., House, D. E., Schreinemachers, D., & Koren, H. S. (1991) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb/4.1.72 · Cited by 470
Metadata
- Authors
- Darlington, G. J., Wilson, D. R., & Lachman, L. B.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1986-09-01
- Volume
- 103
- Issue
- 3
- Pages
- 787-793
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2126726439
- Cited by
- 383
- References
- 0
- FWCI
- 17.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Monocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro. https://app.cochat.ai/papers/10.1083/jcb.103.3.787
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- Darlington, G. J., Wilson, D. R., & Lachman, L. B. (1986). Monocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro. The Journal of cell biology. https://doi.org/10.1083/jcb.103.3.787
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
