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Cited by 193Neuroscience and Neuropharmacology ResearchNeuroinflammation and Neurodegeneration MechanismsNeurogenesis and neuroplasticity mechanisms

Astrotactin: a novel neuronal cell surface antigen that mediates neuron-astroglial interactions in cerebellar microcultures

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Edmondson, JC, Liem, RK, Kuster, JE, Hatten, ME·1988·The Journal of Cell Biology
DOI 10.1083/jcb.106.2.505PMC PMC2114977
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Abstract

A microculture system for mouse cerebellar cells has been used to identify an immune activity, raised in rabbits against postnatal cerebellar cells, that blocks neuron-glial interactions in vitro. In the presence of blocking antibodies, stable neuron-glial contacts did not form and neuronal induction of glial process outgrowth did not occur. Subsequently, neurons were randomly arranged in the cultures rather than organized along the arms of astroglia. We have named the immune activity that blocks neuron-astroglial interactions anti-astrotactin. Partial purification of the anti-astrotactin blocking antibodies was obtained by cellular absorption with PC12 cells, a clonal cell line which expresses both the N-CAM and NILE (Ng-CAM, L1) glycoproteins. Subsequent absorption with purified cerebellar granule cells, but not with astroglial cells, removed the blocking activity, suggesting that the antigen(s) bound by blocking antibodies are neuronal. Immunoprecipitation of [35S]methionine- or [3H]fucose-radiolabeled Triton extracts of early postnatal cerebellar cells showed that the unabsorbed antiserum recognized a large number of proteins. Among these were bands with apparent molecular masses of N-CAM (180 and 140 kD) and NILE (230 kD). After absorption of the immune serum with PC12 cells, the number of bands recognized by the antiserum was reduced to a prominent band at 100 kD and a diffuse smear of material between 80 and 90 kD. The prominent band at 100 kD was removed by subsequent absorption of the immune serum with granule cells, a step which removed the blocking activity in the cerebellar microculture assay. Further evidence suggests that the astrotactin activity is missing or defective on granule cells from the neurological mutant mouse weaver, an animal that suffers a failure of glial-guided neuronal migration. When anti-astrotactin Fab fragments were pre-absorbed with weaver cerebellar neurons and then tested in the functional assay of neuron-glial interactions, the immune blocking activity was not removed. In contrast, wild-type cerebellar neurons removed the anti-astrotactin blocking activity under the same conditions. Subsequently, when [3H]fucose-radiolabeled Triton extracts of weaver and normal cells were immunoprecipitated with whole or PC12-absorbed anti-astrotactin antiserum, the intensity of the band at 100 kD was reduced by 95% in weaver cells.

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  • ASTN2 encodes a protein highly paralogous to ASTN1, which is a mediator of neuronal-glial contact.

    “Astrotactin 2 ( ASTN2 [GenBank accession number AF116574.1]) encodes a protein highly paralogous to ASTN1, which is a mediator of neuronal-glial contact (Edmondson et al. 1988).”

    From Limb-Girdle Muscular Dystrophy Type 2H Associated with Mutation in TRIM32, a Putative E3-Ubiquitin–Ligase Gene (2002) · cites this paper as Edmondson et al. 1988

    The Discussion (p. 515) identifies astrotactin as a neuronal antigen with an apparent role in mediating neuron–astroglial contacts. The paper does not address ASTN2, identify astrotactin as the product of ASTN1, or establish that the two proteins are highly paralogous. (LLM, conf=0.88)

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Authors
Edmondson, J., Liem, R., Kuster, J., & Hatten, M.
Affiliations
  • Columbia University (US)
  • Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1988-02-01
Volume
106
Issue
2
Pages
505-517
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.106.2.505
PMC
PMC2114977
OpenAlex
W2014467217
Cited by
193
References
39
Citations by year
2026: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 3 · 2017: 8 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 2
FWCI
5.24
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
National Institute of Neurological Disorders and Stroke (NS 15429)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
cerebellar granule cells, N-CAM, weaver mouse, PC12 cells, antibody purification, glycoproteins, neuronal adhesion molecules
MeSH terms
Animals, Antigens, Surface, Astrocytes, Cell Adhesion, Cells, Cultured, Cerebellum, Fluorescent Antibody Technique, Immunologic Techniques, Membrane Glycoproteins, Mice, Neurologic Mutants, Molecular Weight, Neurons, Video Recording, Mice

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