Neuronal regulation of astroglial morphology and proliferation in vitro.
Open accessAbstract
To analyze the interdependence of neurons and astroglia during central nervous system development, a rapid method for purifying early postnatal cerebellar neurons and astroglia, and recombining them in vitro, has been developed. The influence of neurons on astroglial shape and proliferation has been evaluated with an in vitro model system previously used to describe the role of cerebellar astroglia in neuronal migration and positioning (Hatten, M. E., and R. K. H. Liem, 1981, J. Cell Biol., 90:622-630; and Hatten, M. E., R. K. H. Liem, and C. A. Mason, 1984, J. Cell Biol., 98:193-204. Cerebellar tissue harvested from C57Bl/6J mouse cerebellum on the third or fourth day postnatal was dissociated into a single cell suspension with trypsin, and enriched glial and neuronal fractions were separated with a step gradient of Percoll. Highly purified astroglial and neuronal fractions resulted from subsequently preplanting the cells on a polylysine-coated culture surface. In the absence of neurons, astroglia, identified by staining with antisera raised against purified glial filament protein, assumed a flattened shape and proliferated rapidly. In the absence of astroglia, cerebellar neurons, identified by staining with antisera raised against the nerve growth factor-inducible large external (NILE) glycoprotein and by electron microscopy, formed cellular reaggregates, had markedly impaired neurite outgrowth, and survived poorly. When purified neurons and isolated astroglia were recombined, astroglial proliferation slowed markedly and the flattened shape expressed in the absence of neurons transformed into highly elongated profiles that resembled embryonic forms of cerebellar astroglia. After longer periods (48-72 h) in the presence of neurons, astroglia had "Bergmann-like" or "astrocyte-like" shapes and neurons commonly associated with them. These results suggest that neurons influence the differentiation of astroglia.
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The proliferation of astrocytes and oligodendrocytes is responsive to trophic cues associated with neuronal activity.
“The proliferation of both these glial cell types is responsive to trophic cues associated with neuronal activity (10–12).”
From Evolution of increased glia–neuron ratios in the human frontal cortex (2006) · cites this paper as [10]
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- Authors
- Hatten, M. E.
- Corresponding author
- Hatten, M E
- ORCID
- Hatten, M E
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1985-02-01
- Volume
- 100
- Issue
- 2
- Pages
- 384-396
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2113456
- OpenAlex
- W2098287644
- Cited by
- 592
- References
- 47
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 5 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 9 · 2019: 9 · 2018: 6 · 2017: 2 · 2016: 10 · 2015: 14 · 2014: 6 · 2013: 16 · 2012: 10
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS 15429)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- neuron-glia interactions, astrocyte proliferation, cerebellar neurons, neuronal differentiation, glial differentiation, neurite outgrowth, Bergmann glia, astrocytes, postnatal cerebellum, cell purification
- MeSH terms
- Animals, Astrocytes, Cell Differentiation, Cell Division, Cell Separation, Cells, Cultured, Cerebellum, Fluorescent Antibody Technique, Mice, Inbred C57BL, Microscopy, Electron, Neurons, Mice
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