The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Neutrophil migration across monolayers of cytokine-prestimulated endothelial cells: a role for platelet-activating factor and IL-8.
Open accessAbstract
In a previous study we observed that neutrophils respond with a rapid rise in [Ca2+]i during adherence to cytokine-activated endothelial cells (EC), caused by EC membrane-associated platelet-activating factor (PAF). In the present study, we investigated whether this form of PAF was important in neutrophil adherence and migration across monolayers of rIL-1 beta- or rTNF alpha-prestimulated EC. PAF receptor antagonists prevented neutrophil migration across cytokine-pretreated EC by approximately 60% (P less than 0.005) without interfering with the process of adherence. The antagonists WEB 2086 and L-652,731 had no effect on neutrophil migration across resting EC induced by formylmethionyl-leucyl-phenylalanine (FMLP). A murine anti-IL-8 antiserum was found to also partially inhibit the neutrophil transmigration across cytokine-activated EC. When the anti-IL-8 antiserum was used in combination with a PAF receptor antagonist, neutrophil migration across cytokine-pretreated monolayers of EC was completely prevented. During transmigration, LAM-1 and CD44 on the neutrophils were down-modulated; both WEB 2086 and anti-IL-8 antiserum partially prevented this down-modulation caused by cytokine-prestimulated EC. Our results indicate that human neutrophils are activated and guided by EC-associated PAF and EC-derived IL-8 during the in vitro diapedesis in between cytokine-stimulated EC.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Adhesion receptors of the immune system
Springer, T. A. (1990) · Nature · doi:10.1038/346425a0 · Cited by 6,477
Cited by
T-cell adhesion induced by proteoglycan-immobilized cytokine MIP-lβ
Tanaka, Y., Adams, D. H., Hubscher, S., Hirano, H., Siebenlist, U., & Shaw, S. (1993) · Nature · doi:10.1038/361079a0 · Cited by 873
Role of Platelet-Activating Factor in Cardiovascular Pathophysiology
Montrucchio, G., Alloatti, G., & Camussi, G. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.4.1669 · Cited by 371
Leukocyte—endothelial cell interactions
McEver, R. P. (1992) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(92)90109-p · Cited by 128
Nasopoulou, C., Karantonis, H. C., Detopoulou, M., Demopoulos, C. A., & Zabetakis, I. (2014) · Phytochemistry Reviews · doi:10.1007/s11101-014-9350-8 · Cited by 26
Metadata
- Authors
- Kuijpers, T. W., Hakkert, B. C., Hart, M. H., & Roos, D.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1992-05-01
- Volume
- 117
- Issue
- 3
- Pages
- 565-572
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2031500043
- Cited by
- 203
- References
- 2
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Neutrophil migration across monolayers of cytokine-prestimulated endothelial cells: a role for platelet-activating factor and IL-8. https://app.cochat.ai/papers/10.1083/jcb.117.3.565
- The paper
- Kuijpers, T. W., Hakkert, B. C., Hart, M. H., & Roos, D. (1992). Neutrophil migration across monolayers of cytokine-prestimulated endothelial cells: a role for platelet-activating factor and IL-8. The Journal of cell biology. https://doi.org/10.1083/jcb.117.3.565
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
