The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton.
Open accessAbstract
By immunogold labeling, we demonstrate that "millipede-like" structures seen previously in mammalian cell cytoskeletons after removal of actin by treatment with gelsolin are composed of the cores of vimentin IFs with sidearms containing plectin. These plectin sidearms connect IFs to microtubules, the actin-based cytoskeleton and possibly membrane components. Plectin binding to microtubules was significantly increased in cells from transgenic mice lacking IFs and was reversed by microinjection of exogenous vimentin. These results suggest the existence of a pool of plectin which preferentially associates with IFs but may also be competed for by microtubules. The association of IFs with microtubules did not show a preference for Glu-tubulin. Nor did it depend upon the presence of MAP4 since plectin links were retained after specific immunodepletion of MAP4. The association of IFs with stress fibers survived actin depletion by gelsolin suggesting that myosin II minifilaments or components closely associated with them may play a role as plectin targets. Our results provide direct structural evidence for the hypothesis that plectin cross-links elements of the cytoskeleton thus leading to integration of the cytoplasm.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton
Burridge, K., Fath, K., Kelly, T., Nuckolls, G., & Turner, C. (1988) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.04.110188.002415 · Cited by 2,120
INTERMEDIATE FILAMENTS: Structure, Dynamics, Function and Disease
Fuchs, E., & Weber, K. (1994) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.63.070194.002021 · Cited by 1,411
Structural interaction of cytoskeletal components
Schliwa, M., & van Blerkom, J. (1981) · The Journal of Cell Biology · doi:10.1083/jcb.90.1.222 · Cited by 756
Hirokawa, N. (1982) · The Journal of Cell Biology · doi:10.1083/jcb.94.1.129 · Cited by 671
Mice lacking vimentin develop and reproduce without an obvious phenotype
Colucci-Guyon, E., Portier, M.-M., Dunia, I., Paulin, D., Pournin, S., & Babinet, C. (1994) · Cell · doi:10.1016/0092-8674(94)90553-3 · Cited by 598
Gundersen, G. G., Kalnoski, M. H., & Bulinski, J. C. (1984) · Cell · doi:10.1016/0092-8674(84)90273-3 · Cited by 533
MITOSIS AND INTERMEDIATE-SIZED FILAMENTS IN DEVELOPING SKELETAL MUSCLE
Ishikawa, H., Bischoff, R., & Holtzer, H. (1968) · The Journal of Cell Biology · doi:10.1083/jcb.38.3.538 · Cited by 517
Plectin deficiency results in muscular dystrophy with epidermolysis bullosa
Smith, F. J. D., Eady, R. A. J., Leigh, I. M., McMillan, J. R., Rugg, E. L., Kelsell, D. P., Bryant, S. P., Spurr, N. K., Geddes, J. F., Kirtschig, G., et al. (1996) · Nature Genetics · doi:10.1038/ng0896-450 · Cited by 388
Verkhovsky, A. B., Svitkina, T. M., & Borisy, G. G. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.4.989 · Cited by 324
Self-citationMcLean, W. H., Pulkkinen, L., Smith, F. J., Rugg, E. L., Lane, E. B., Bullrich, F., Burgeson, R. E., Amano, S., Hudson, D. L., Owaribe, K., et al. (1996) · Genes & Development · doi:10.1101/gad.10.14.1724 · Cited by 303
Cited by
Neurofilaments and Neurofilament Proteins in Health and Disease
Yuan, A., Rao, M. V., Veeranna, & Nixon, R. A. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a018309 · Cited by 731
Svitkina, T. M., Verkhovsky, A. B., McQuade, K. M., & Borisy, G. G. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.139.2.397 · Cited by 718
Self-citationPark, J. E., Tan, H. S., Datta, A., Lai, R. C., Zhang, H., Meng, W., Lim, S. K., & Sze, S. K. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m900381-mcp200 · Cited by 582
Intermediate filaments mediate cytoskeletal crosstalk
Chang, L., & Goldman, R. D. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1438 · Cited by 386
The role of the cytoskeleton in heart failure
Hein, S. (2000) · Cardiovascular Research · doi:10.1016/s0008-6363(99)00268-0 · Cited by 353
Metadata
- Authors
- Svitkina, T. M., Verkhovsky, A. B., & Borisy, G. G.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1996-11-15
- Volume
- 135
- Issue
- 4
- Pages
- 991-1007
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2144880676
- Cited by
- 414
- References
- 44
- FWCI
- 8.96
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton. https://app.cochat.ai/papers/10.1083/jcb.135.4.991
- The paper
- Svitkina, T. M., Verkhovsky, A. B., & Borisy, G. G. (1996). Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton. The Journal of cell biology. https://doi.org/10.1083/jcb.135.4.991
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
