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Targeting of Protein Kinase Cα to Caveolae
Open accessAbstract
Previously, we showed caveolae contain a population of protein kinase Cα (PKCα) that appears to regulate membrane invagination. We now report that multiple PKC isoenzymes are enriched in caveolae of unstimulated fibroblasts. To understand the mechanism of PKC targeting, we prepared caveolae lacking PKCα and measured the interaction of recombinant PKCα with these membranes. PKCα bound with high affinity and specificity to caveolae membranes. Binding was calcium dependent, did not require the addition of factors that activate the enzyme, and involved the regulatory domain of the molecule. A 68-kD PKCα-binding protein identified as sdr (serum deprivation response) was isolated by interaction cloning and localized to caveolae. Antibodies against sdr inhibited PKCα binding. A 100–amino acid sequence from the middle of sdr competitively blocked PKCα binding while flanking sequences were inactive. Caveolae appear to be a membrane site where PKC enzymes are organized to carry out essential regulatory functions as well as to modulate signal transduction at the cell surface.
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Citations
Cited by
Structural Basis of Protein Kinase C Isoform Function
Steinberg, S. F. (2008) · Physiological Reviews · doi:10.1152/physrev.00034.2007 · Cited by 872
Neurogenic inflammation after traumatic brain injury and its potentiation of classical inflammation
Corrigan, F., Mander, K. A., Leonard, A. V., & Vink, R. (2016) · Journal of Neuroinflammation · doi:10.1186/s12974-016-0738-9 · Cited by 331
Caveolae, caveolins, and cavins: complex control of cellular signalling and inflammation
Chidlow, J. H., & Sessa, W. C. (2010) · Cardiovascular Research · doi:10.1093/cvr/cvq075 · Cited by 310
Exploring the caves: cavins, caveolins and caveolae
Hansen, C. G., & Nichols, B. J. (2010) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.01.005 · Cited by 295
SDPR induces membrane curvature and functions in the formation of caveolae
Hansen, C. G., Bright, N. A., Howard, G., & Nichols, B. J. (2009) · Nature Cell Biology · doi:10.1038/ncb1887 · Cited by 257
Metadata
- Authors
- Mineo, C., Ying, Y.-S., Chapline, C., Jaken, S., & Anderson, R. G. W.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1998-05-04
- Volume
- 141
- Issue
- 3
- Pages
- 601-610
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W1923109334
- Cited by
- 186
- References
- 0
- FWCI
- 3.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Mineo, C., Ying, Y.-S., Chapline, C., Jaken, S., & Anderson, R. G. W. (1998). Targeting of Protein Kinase Cα to Caveolae. The Journal of Cell Biology. https://doi.org/10.1083/jcb.141.3.601
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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