The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Sulfotransferases of Two Specificities Function in the Reconstitution of High Endothelial Cell Ligands for L-selectin
Open accessAbstract
L-selectin, a lectin-like receptor, mediates rolling of lymphocytes on high endothelial venules (HEVs) in secondary lymphoid organs by interacting with HEV ligands. These ligands consist of a complex of sialomucins, candidates for which are glycosylation- dependent cell adhesion molecule 1 (GlyCAM-1), CD34, and podocalyxin. The ligands must be sialylated, fucosylated, and sulfated for optimal recognition by L-selectin. Our previous structural characterization of GlyCAM-1 has demonstrated two sulfation modifications, Gal-6-sulfate and GlcNAc-6-sulfate in the context of sialyl Lewis x. We now report the cloning of a Gal-6-sulfotransferase and a GlcNAc-6-sulfotransferase, which can modify GlyCAM-1 and CD34. The Gal-6-sulfotransferase shows a wide tissue distribution. In contrast, the GlcNAc-6-sulfotransferase is highly restricted to HEVs, as revealed by Northern analysis and in situ hybridization. Expression of either enzyme in Chinese hamster ovary cells, along with CD34 and fucosyltransferase VII, results in ligand activity, as detected by binding of an L-selectin/IgM chimera. When coexpressed, the two sulfotransferases synergize to produce strongly enhanced chimera binding.
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Cites
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Cited by
A Genetic Approach to Mammalian Glycan Function
Lowe, J. B., & Marth, J. D. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161809 · Cited by 594
Sulfotransferases: Structure, Mechanism, Biological Activity, Inhibition, and Synthetic Utility
Chapman, E., Best, M. D., Hanson, S. R., & Wong, C. (2004) · Angewandte Chemie International Edition · doi:10.1002/anie.200300631 · Cited by 543
Glycan Array Screening Reveals a Candidate Ligand for Siglec-8*
Bochner, B. S., Alvarez, R. A., Mehta, P., Bovin, N. V., Blixt, O., White, J. R., & Schnaar, R. L. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m412378200 · Cited by 262
Kanda, H., Newton, R., Klein, R., Morita, Y., Gunn, M. D., & Rosen, S. D. (2008) · Nature Immunology · doi:10.1038/ni1573 · Cited by 257
Self-citationKawashima, H., Atarashi, K., Hirose, M., Hirose, J., Yamada, S., Sugahara, K., & Miyasaka, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200396200 · Cited by 241
Metadata
- Authors
- Bistrup, A., Bhakta, S., Lee, J. K., Belov, Y. Y., Gunn, M. D., Zuo, F.-R., Huang, C.-C., Kannagi, R., Rosen, S. D., & Hemmerich, S.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-05-17
- Volume
- 145
- Issue
- 4
- Pages
- 899-910
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2001091272
- Cited by
- 258
- References
- 5
- FWCI
- 10.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Bistrup, A., Bhakta, S., Lee, J. K., Belov, Y. Y., Gunn, M. D., Zuo, F.-R., Huang, C.-C., Kannagi, R., Rosen, S. D., & Hemmerich, S. (1999). Sulfotransferases of Two Specificities Function in the Reconstitution of High Endothelial Cell Ligands for L-selectin. The Journal of Cell Biology. https://doi.org/10.1083/jcb.145.4.899
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