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Cited by 241Proteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins ResearchCell Adhesion Molecules Research

Oversulfated Chondroitin/Dermatan Sulfates Containing GlcAβ1/IdoAα1–3GalNAc(4,6-O-disulfate) Interact with L- and P-selectin and Chemokines

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Kawashima, Hiroto, Atarashi, Kazuyuki, Hirose, Mayumi, Hirose, Jun, Yamada, Shuhei, Sugahara, Kazuyuki, Miyasaka, Masayuki·2002·Journal of Biological Chemistry
DOI 10.1074/jbc.m200396200
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Abstract

We previously reported that versican, a large chondroitin/dermatan sulfate (CS/DS) proteoglycan, interacts through its CS/DS chains with adhesion molecules L- and P-selectin and CD44, as well as chemokines. Here, we have characterized these interactions further. Using a metabolic inhibitor of sulfation, sodium chlorate, we show that the interactions of the CS/DS chains of versican with L- and P-selectin and chemokines are sulfation-dependent but the interaction with CD44 is sulfation-independent. Consistently, versican's binding to L- and P-selectin and chemokines is specifically inhibited by oversulfated CS/DS chains containing GlcAbeta1-3GalNAc(4,6-O-disulfate) or IdoAalpha1-3GalNAc(4,6-O-disulfate), but its binding to CD44 is inhibited by all the CS/DS chains, including low-sulfated and unsulfated ones. Affinity and kinetic analyses using surface plasmon resonance revealed that the oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) bind directly to selectins and chemokines with high affinity (K(d) 21.1 to 293 nm). In addition, a tetrasaccharide fragment of repeating GlcAbeta1-3GalNAc(4,6-O-disulfate) units directly interacts with L- and P-selectin and chemokines and oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) inhibit chemokine-induced Ca(2+) mobilization. Taken together, our results show that oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) are recognized by L- and P-selectin and chemokines, and imply that these chains are important in selectin- and/or chemokine-mediated cellular responses.

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  • As a consequence of their antiselectin activity, these polysaccharides attenuated metastasis and inflammation.

    “As a consequence of their antiselectin activity, these polysaccharides attenuated metastasis and inflammation [150,151,152].”

    From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [151]

  • Some polysaccharides from marine animals inhibited the binding of pro-inflammatory molecules P- and L-selectins to immobilized carbohydrate determinant sialyl Lewisx.

    “Some polysaccharides from the marine animals inhibited the binding of pro-inflammatory molecules, P- and L-selectins, to immobilized carbohydrate determinant sialyl Lewisx which is a component of cell surface glycoproteins presented in leukocytes and overexpressed in several tumor cells.”

    From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [151]

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Authors
Kawashima, H., Atarashi, K., Hirose, M., Hirose, J., Yamada, S., Sugahara, K., & Miyasaka, M.
Affiliations
  • The University of Osaka (JP)
  • Kobe Pharmaceutical University (JP)
Corresponding author
Kawashima, Hiroto
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2002-04
Volume
277
Issue
15
Pages
12921-12930
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m200396200
OpenAlex
W2017704712
Cited by
241
References
40
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 8 · 2021: 6 · 2020: 7 · 2019: 5 · 2018: 8 · 2017: 9 · 2016: 6 · 2015: 8 · 2014: 13 · 2013: 17 · 2012: 11
FWCI
6.43
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
versican, L-selectin, P-selectin, CD44, chemokine binding, calcium mobilization, cell adhesion
MeSH terms
Base Sequence, Chondroitin Sulfates, Dermatan Sulfate, Humans, Kinetics, Oligosaccharides, Protein Binding, DNA Primers, P-Selectin, L-Selectin

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