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Structure and Assembly of the Nup84p Complex
Open accessAbstract
The Nup84p complex consists of five nucleoporins (Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p) and Sec13p, a bona fide subunit of the COPII coat complex. We show that a pool of green fluorescent protein–tagged Sec13p localizes to the nuclear pores in vivo, and identify sec13 mutant alleles that are synthetically lethal with nup85Δ and affect the localization of a green fluorescent protein–Nup49p reporter protein. In the electron microscope, sec13 mutants exhibit structural defects in nuclear pore complex (NPC) and nuclear envelope organization. For the assembly of the complex, Nup85p, Nup120p, and Nup145p-C are essential. A highly purified Nup84p complex was isolated from yeast under native conditions and its molecular mass was determined to be 375 kD by quantitative scanning transmission electron microscopy and analytical ultracentrifugation, consistent with a monomeric complex. Furthermore, the Nup84p complex exhibits a Y-shaped, triskelion-like morphology 25 nm in diameter in the transmission electron microscope. Thus, the Nup84p complex constitutes a paradigm of an NPC structural module with distinct composition, structure, and a role in nuclear mRNA export and NPC bio- genesis.
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Citations
Cited by
The Nuclear Pore Complex and Nuclear Transport
Wente, S. R., & Rout, M. P. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000562 · Cited by 686
The Structure of the Nuclear Pore Complex (An Update)
Lin, D. H., & Hoelz, A. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-062917-011901 · Cited by 478
The Nuclear Pore Complex as a Flexible and Dynamic Gate
Knockenhauer, K. E., & Schwartz, T. U. (2016) · Cell · doi:10.1016/j.cell.2016.01.034 · Cited by 406
Components of Coated Vesicles and Nuclear Pore Complexes Share a Common Molecular Architecture
Devos, D., Dokudovskaya, S., Alber, F., Williams, R., Chait, B. T., Sali, A., & Rout, M. P. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020380 · Cited by 391
AI-based structure prediction empowers integrative structural analysis of human nuclear pores
Mosalaganti, S., Obarska-Kosinska, A., Siggel, M., Taniguchi, R., Turoňová, B., Zimmerli, C. E., Buczak, K., Schmidt, F. H., Margiotta, E., Mackmull, M.-T., et al. (2022) · Science · doi:10.1126/science.abm9506 · Cited by 355
Metadata
- Authors
- Siniossoglou, S., Lutzmann, M., Santos-Rosa, H., Leonard, K., Mueller, S., Aebi, U., & Hurt, E.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-04-03
- Volume
- 149
- Issue
- 1
- Pages
- 41-54
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2140235777
- Cited by
- 168
- References
- 0
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Siniossoglou, S., Lutzmann, M., Santos-Rosa, H., Leonard, K., Mueller, S., Aebi, U., & Hurt, E. (2000). Structure and Assembly of the Nup84p Complex. The Journal of Cell Biology. https://doi.org/10.1083/jcb.149.1.41
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