The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Calreticulin Is a Receptor for Nuclear Export
Open accessAbstract
In previous work, we used a permeabilized cell assay that reconstitutes nuclear export of protein kinase inhibitor (PKI) to show that cytosol contains an export activity that is distinct from Crm1 (Holaska, J.M., and B.M. Paschal. 1995. Proc. Natl. Acad. Sci. USA. 95: 14739–14744). Here, we describe the purification and characterization of the activity as calreticulin (CRT), a protein previously ascribed to functions in the lumen of the ER. We show that cells contain both ER and cytosolic pools of CRT. The mechanism of CRT-dependent export of PKI requires a functional nuclear export signal (NES) in PKI and involves formation of an export complex that contains RanGTP. Previous studies linking CRT to downregulation of steroid hormone receptor function led us to examine its potential role in nuclear export of the glucocorticoid receptor (GR). We found that CRT mediates nuclear export of GR in permeabilized cell, microinjection, and transfection assays. GR export is insensitive to the Crm1 inhibitor leptomycin B in vivo, and it does not rely on a leucine-rich NES. Rather, GR export is facilitated by its DNA-binding domain, which is shown to function as an NES when transplanted to a green fluorescent protein reporter. CRT defines a new export pathway that may regulate the transcriptional activity of steroid hormone receptors.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
CRM1 Is an Export Receptor for Leucine-Rich Nuclear Export Signals
Fornerod, M., Ohno, M., Yoshida, M., & Mattaj, I. W. (1997) · Cell · doi:10.1016/s0092-8674(00)80371-2 · Cited by 2,081
Transport Between the Cell Nucleus and the Cytoplasm
Görlich, D., & Kutay, U. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.607 · Cited by 2,022
Identification of a signal for rapid export of proteins from the nucleus
Wen, W., Meinkotht, J. L., Tsien, R. Y., & Taylor, S. S. (1995) · Cell · doi:10.1016/0092-8674(95)90435-2 · Cited by 1,190
Exportin 1 (Crm1p) Is an Essential Nuclear Export Factor
Stade, K., Ford, C. S., Guthrie, C., & Weis, K. (1997) · Cell · doi:10.1016/s0092-8674(00)80370-0 · Cited by 1,141
Fischer, U., Huber, J., Boelens, W. C., Mattajt, L. W., & Lührmann, R. (1995) · Cell · doi:10.1016/0092-8674(95)90436-0 · Cited by 1,139
Kudo, N., Matsumori, N., Taoka, H., Fujiwara, D., Schreiner, E. P., Wolff, B., Yoshida, M., & Horinouchi, S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.16.9112 · Cited by 1,029
Transport of Proteins and RNAs in and out of the Nucleus
Nakielny, S., & Dreyfuss, G. (1999) · Cell · doi:10.1016/s0092-8674(00)81666-9 · Cited by 755
Export of Importin α from the Nucleus Is Mediated by a Specific Nuclear Transport Factor
Kutay, U., Bischoff, F. R., Kostka, S., Kraft, R., & Görlich, D. (1997) · Cell · doi:10.1016/s0092-8674(00)80372-4 · Cited by 618
The Translocon: A Dynamic Gateway at the ER Membrane
Johnson, A. E., & van Waes, M. A. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.799 · Cited by 603
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
Grüter, P., Tabernero, C., von Kobbe, C., Schmitt, C., Saavedra, C., Bachi, A., Wilm, M., Felber, B. K., & Izaurralde, E. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80065-9 · Cited by 560
Cited by
Immune regulation by glucocorticoids
Cain, D. W., & Cidlowski, J. A. (2017) · Nature Reviews Immunology · doi:10.1038/nri.2017.1 · Cited by 1,846
Role of hsp90 and the hsp90-binding immunophilins in signalling protein movement
Pratt, W. B., Galigniana, M. D., Harrell, J. M., & DeFranco, D. B. (2004) · Cellular Signalling · doi:10.1016/j.cellsig.2004.02.004 · Cited by 285
Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis
Müller‐Taubenberger, A., Lupas, A. N., Li, H., Ecke, M., Simmeth, E., & Gerisch, G. (2001) · The EMBO Journal · doi:10.1093/emboj/20.23.6772 · Cited by 242
The significance of glucocorticoid pulsatility
Lightman, S. L., Wiles, C. C., Atkinson, H. C., Henley, D. E., Russell, G. M., Leendertz, J. A., McKenna, M. A., Spiga, F., Wood, S. A., & Conway-Campbell, B. L. (2008) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2007.11.073 · Cited by 241
Conway-Campbell, B. L., McKenna, M. A., Wiles, C. C., Atkinson, H. C., de Kloet, E. R., & Lightman, S. L. (2007) · Endocrinology · doi:10.1210/en.2007-0585 · Cited by 125
Metadata
- Authors
- Holaska, J. M., Black, B. E., Love, D. C., Hanover, J. A., Leszyk, J., & Paschal, B. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-01-08
- Volume
- 152
- Issue
- 1
- Pages
- 127-140
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W1967658150
- Cited by
- 277
- References
- 55
- FWCI
- 9.66
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Calreticulin Is a Receptor for Nuclear Export. https://app.cochat.ai/papers/10.1083/jcb.152.1.127
- The paper
- Holaska, J. M., Black, B. E., Love, D. C., Hanover, J. A., Leszyk, J., & Paschal, B. M. (2001). Calreticulin Is a Receptor for Nuclear Export. The Journal of Cell Biology. https://doi.org/10.1083/jcb.152.1.127
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
