The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Rho-Kinase–Mediated Contraction of Isolated Stress Fibers
Open accessAbstract
It is widely accepted that actin filaments and the conventional double-headed myosin interact to generate force for many types of nonmuscle cell motility, and that this interaction occurs when the myosin regulatory light chain (MLC) is phosphorylated by MLC kinase (MLCK) together with calmodulin and Ca2+. However, recent studies indicate that Rho-kinase is also involved in regulating the smooth muscle and nonmuscle cell contractility. We have recently isolated reactivatable stress fibers from cultured cells and established them as a model system for actomyosin-based contraction in nonmuscle cells. Here, using isolated stress fibers, we show that Rho-kinase mediates MLC phosphorylation and their contraction in the absence of Ca2+. More rapid and extensive stress fiber contraction was induced by MLCK than was by Rho-kinase. When the activity of Rho-kinase but not MLCK was inhibited, cells not only lost their stress fibers and focal adhesions but also appeared to lose cytoplasmic tension. Our study suggests that actomyosin-based nonmuscle contractility is regulated by two kinase systems: the Ca2+-dependent MLCK and the Rho-kinase systems. We propose that Ca2+ is used to generate rapid contraction, whereas Rho-kinase plays a major role in maintaining sustained contraction in cells.
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Cites
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Regulation of Myosin Phosphatase by Rho and Rho-Associated Kinase (Rho-Kinase)
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Self-citationCalcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension
Uehata, M., Ishizaki, T., Satoh, H., Ono, T., Kawahara, T., Morishita, T., Tamakawa, H., Yamagami, K., Inui, J., Maekawa, M., et al. (1997) · Nature · doi:10.1038/40187 · Cited by 2,859
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Phosphorylation and Activation of Myosin by Rho-associated Kinase (Rho-kinase)
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Self-citationTENSEGRITY: THE ARCHITECTURAL BASIS OF CELLULAR MECHANOTRANSDUCTION
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Cited by
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Tomasek, J. J., Gabbiani, G., Hinz, B., Chaponnier, C., & Brown, R. A. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm809 · Cited by 4,290
Environmental sensing through focal adhesions
Geiger, B., Spatz, J. P., & Bershadsky, A. D. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2593 · Cited by 2,543
ROCKs: multifunctional kinases in cell behaviour
Riento, K., & Ridley, A. J. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1128 · Cited by 1,941
Rho‐kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
Amano, M., Nakayama, M., & Kaibuchi, K. (2010) · Cytoskeleton · doi:10.1002/cm.20472 · Cited by 994
Self-citationActin stress fibers – assembly, dynamics and biological roles
Tojkander, S., Gateva, G., & Lappalainen, P. (2012) · Journal of Cell Science · doi:10.1242/jcs.098087 · Cited by 900
Metadata
- Authors
- Katoh, K., Kano, Y., Amano, M., Onishi, H., Kaibuchi, K., & Fujiwara, K.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-04-30
- Volume
- 153
- Issue
- 3
- Pages
- 569-584
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2158253639
- Cited by
- 331
- References
- 49
- FWCI
- 6.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Katoh, K., Kano, Y., Amano, M., Onishi, H., Kaibuchi, K., & Fujiwara, K. (2001). Rho-Kinase–Mediated Contraction of Isolated Stress Fibers. The Journal of Cell Biology. https://doi.org/10.1083/jcb.153.3.569
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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