The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Marching at the front and dragging behind
Open accessAbstract
Integrins are cell–substrate adhesion molecules that provide the essential link between the actin cytoskeleton and the extracellular matrix during cell migration. We have analyzed αVβ3-integrin dynamics in migrating cells using a green fluorescent protein–tagged β3-integrin chain. At the cell front, adhesion sites containing αVβ3-integrin remain stationary, whereas at the rear of the cell they slide inward. The integrin fluorescence intensity within these different focal adhesions, and hence the relative integrin density, is directly related to their mobility. Integrin density is as much as threefold higher in sliding compared with stationary focal adhesions. High intracellular tension under the control of RhoA induced the formation of high-density contacts. Low-density adhesion sites were induced by Rac1 and low intracellular tension. Photobleaching experiments demonstrated a slow turnover of β3-integrins in low-density contacts, which may account for their stationary nature. In contrast, the fast β3-integrin turnover observed in high-density contacts suggests that their apparent sliding may be caused by a polarized renewal of focal contacts. Therefore, differential acto-myosin–dependent integrin turnover and focal adhesion densities may explain the mechanical and behavioral differences between cell adhesion sites formed at the front, and those that move in the retracting rear of migrating cells.
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Cites
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Cited by
Tumour-cell invasion and migration: diversity and escape mechanisms
Friedl, P., & Wolf, K. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1075 · Cited by 3,222
Environmental sensing through focal adhesions
Geiger, B., Spatz, J. P., & Bershadsky, A. D. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2593 · Cited by 2,543
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T., et al. (2010) · Nature · doi:10.1038/nature09198 · Cited by 1,541
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Vinculin controls focal adhesion formation by direct interactions with talin and actin
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Self-citation
Metadata
- Authors
- Ballestrem, C., Hinz, B., Imhof, B. A., & Wehrle-Haller, B.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-12-24
- Volume
- 155
- Issue
- 7
- Pages
- 1319-1332
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2147023756
- Cited by
- 369
- References
- 65
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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