The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis
Open accessAbstract
Aurora B is a mitotic protein kinase that phosphorylates histone H3, behaves as a chromosomal passenger protein, and functions in cytokinesis. We investigated a role for Aurora B with respect to human centromere protein A (CENP-A), a centromeric histone H3 homologue. Aurora B concentrates at centromeres in early G2, associates with histone H3 and centromeres at the times when histone H3 and CENP-A are phosphorylated, and phosphorylates histone H3 and CENP-A in vitro at a similar target serine residue. Dominant negative phosphorylation site mutants of CENP-A result in a delay at the terminal stage of cytokinesis (cell separation). The only molecular defects detected in analysis of 22 chromosomal, spindle, and regulatory proteins were disruptions in localization of inner centromere protein (INCENP), Aurora B, and a putative partner phosphatase, PP1γ1. Our data support a model where CENP-A phosphorylation is involved in regulating Aurora B, INCENP, and PP1γ1 targeting within the cell. These experiments identify an unexpected role for the kinetochore in regulation of cytokinesis.
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Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943 · cited 1×
“Cell lines and culture conditions HeLa TTA cells (Gossen and Bujard, 1992) and derivations thereof were cultured at 37 C in DME medium containing 10% fetal calf serum (GIBCO BRL) with 5% CO2 in the presence of 1 g/ml tetracycline and geneticin (400 g/ml; GIBCO BRL).”
MethodCyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts
Pagano, M., Theodoras, A. M., Tam, S. W., & Draetta, G. F. (1994) · Genes & Development · doi:10.1101/gad.8.14.1627 · Cited by 294
Cited by
Harrington, E. A., Bebbington, D., Moore, J., Rasmussen, R. K., Ajose-Adeogun, A. O., Nakayama, T., Graham, J. A., Demur, C., Hercend, T., Diu-Hercend, A., et al. (2004) · Nature Medicine · doi:10.1038/nm1003 · Cited by 965
The molecular basis for centromere identity and function
McKinley, K. L., & Cheeseman, I. M. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.5 · Cited by 668
The Aurora kinases: Role in cell transformation and tumorigenesis
Katayama, H., Brinkley, W. R., & Sen, S. (2003) · Cancer and Metastasis Reviews · doi:10.1023/a:1023789416385 · Cited by 398
Aurora kinases: novel therapy targets in cancers
Tang, A., Gao, K., Chu, L., Zhang, R., Yang, J., & Zheng, J. (2017) · Oncotarget · doi:10.18632/oncotarget.14893 · Cited by 382
Sensing centromere tension: Aurora B and the regulation of kinetochore function
Lampson, M. A., & Cheeseman, I. M. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.10.007 · Cited by 375
Metadata
- Authors
- Zeitlin, S. G., Shelby, R. D., & Sullivan, K. F.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-12-24
- Volume
- 155
- Issue
- 7
- Pages
- 1147-1158
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2125754609
- Cited by
- 330
- References
- 2
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- green
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