The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Human TPX2 is required for targeting Aurora-A kinase to the spindle
Open accessAbstract
Aurora-A is a serine-threonine kinase implicated in the assembly and maintenance of the mitotic spindle. Here we show that human Aurora-A binds to TPX2, a prominent component of the spindle apparatus. TPX2 was identified by mass spectrometry as a major protein coimmunoprecipitating specifically with Aurora-A from mitotic HeLa cell extracts. Conversely, Aurora-A could be detected in TPX2 immunoprecipitates. This indicates that subpopulations of these two proteins undergo complex formation in vivo. Binding studies demonstrated that the NH2 terminus of TPX2 can directly interact with the COOH-terminal catalytic domain of Aurora-A. Although kinase activity was not required for this interaction, TPX2 was readily phosphorylated by Aurora-A. Upon siRNA-mediated elimination of TPX2 from cells, the association of Aurora-A with the spindle microtubules was abolished, although its association with spindle poles was unaffected. Conversely, depletion of Aurora-A by siRNA had no detectable influence on the localization of TPX2. We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly.
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Cites
Giet, R., Uzbekov, R., Cubizolles, F., Le Guellec, K., & Prigent, C. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.15005 · Cited by 219
Cited by
Harrington, E. A., Bebbington, D., Moore, J., Rasmussen, R. K., Ajose-Adeogun, A. O., Nakayama, T., Graham, J. A., Demur, C., Hercend, T., Diu-Hercend, A., et al. (2004) · Nature Medicine · doi:10.1038/nm1003 · Cited by 965
Shared and separate functions of polo-like kinases and aurora kinases in cancer
Lens, S. M. A., Voest, E. E., & Medema, R. H. (2010) · Nature Reviews Cancer · doi:10.1038/nrc2964 · Cited by 597
Aurora A kinase (AURKA) in normal and pathological cell division
Nikonova, A. S., Astsaturov, I., Serebriiskii, I. G., Dunbrack, R. L., & Golemis, E. A. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1073-7 · Cited by 448
Aurora-A: the maker and breaker of spindle poles
Barr, A. R., & Gergely, F. (2007) · Journal of Cell Science · doi:10.1242/jcs.013136 · Cited by 441
The Aurora kinase family in cell division and cancer
Vader, G., & Lens, S. M. A. (2008) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2008.07.003 · Cited by 417
Metadata
- Authors
- Kufer, T. A., Silljé, H. H. W., Körner, R., Gruss, O. J., Meraldi, P., & Nigg, E. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2002-08-12
- Volume
- 158
- Issue
- 4
- Pages
- 617-623
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W815233466
- Cited by
- 587
- References
- 1
- FWCI
- 8.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Kufer, T. A., Silljé, H. H. W., Körner, R., Gruss, O. J., Meraldi, P., & Nigg, E. A. (2002). Human TPX2 is required for targeting Aurora-A kinase to the spindle. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200204155
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