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SUMO-2/3 regulates topoisomerase II in mitosis
Open accessAbstract
We have analyzed the abundance of SUMO-conjugated species during the cell cycle in Xenopus egg extracts. The predominant SUMO conjugation products associated with mitotic chromosomes arose from SUMO conjugation of topoisomerase II. Topoisomerase II was modified exclusively by SUMO-2/3 during mitosis under normal circumstances, although we observed conjugation of topoisomerase II to SUMO-1 in extracts with exogenous SUMO-1 protein. Inhibition of SUMO modification by a dominant-negative mutant of the SUMO-conjugating enzyme Ubc9 (dnUbc9) did not detectably alter topoisomerase II activity, but it did increase the amount of unmodified topoisomerase II retained on mitotic chromosomes after high salt washing. dnUbc9 did not disrupt the assembly of condensed mitotic chromosomes or block progression of extracts through mitosis, but it did block the dissociation of sister chromatids at the metaphase–anaphase transition. Together, our results suggest that SUMO conjugation is important for chromosome segregation in metazoan systems, and that mobilization of topoisomerase II from mitotic chromatin may be a key target of this modification.
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Cited by
New Mechanistic and Functional Insights into DNA Topoisomerases
Chen, S. H., Chan, N.-L., & Hsieh, T.-S. (2013) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061809-100002 · Cited by 369
Lee, Y.-J., Miyake, S.-I., Wakita, H., McMullen, D. C., Azuma, Y., Auh, S., & Hallenbeck, J. M. (2007) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600395 · Cited by 202
Self-citationXU, Z., & AU, S. W. N. (2005) · Biochemical Journal · doi:10.1042/bj20041210 · Cited by 79
Reprogramming to pluripotency can conceal somatic cell chromosomal instability
Hamada, M., Malureanu, L. A., Wijshake, T., Zhou, W., & van Deursen, J. M. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002913 · Cited by 16
Metadata
- Authors
- Azuma, Y., Arnaoutov, A., & Dasso, M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-11-03
- Volume
- 163
- Issue
- 3
- Pages
- 477-487
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2168796310
- Cited by
- 199
- References
- 0
- FWCI
- 4.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Azuma, Y., Arnaoutov, A., & Dasso, M. (2003). SUMO-2/3 regulates topoisomerase II in mitosis. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200304088
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