The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
An actin molecular treadmill and myosins maintain stereocilia functional architecture and self-renewal
Open accessAbstract
We have previously shown that the seemingly static paracrystalline actin core of hair cell stereocilia undergoes continuous turnover. Here, we used the same approach of transfecting hair cells with actin–green fluorescent protein (GFP) and espin-GFP to characterize the turnover process. Actin and espin are incorporated at the paracrystal tip and flow rearwards at the same rate. The flux rates (∼0.002–0.04 actin subunits s−1) were proportional to the stereocilia length so that the entire staircase stereocilia bundle was turned over synchronously. Cytochalasin D caused stereocilia to shorten at rates matching paracrystal turnover. Myosins VI and VIIa were localized alongside the actin paracrystal, whereas myosin XVa was observed at the tips at levels proportional to stereocilia lengths. Electron microscopy analysis of the abnormally short stereocilia in the shaker 2 mice did not show the characteristic tip density. We argue that actin renewal in the paracrystal follows a treadmill mechanism, which, together with the myosins, dynamically shapes the functional architecture of the stereocilia bundle.
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Metadata
- Authors
- Rzadzinska, A. K., Schneider, M. E., Davies, C., Riordan, G. P., & Kachar, B.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2004-03-15
- Volume
- 164
- Issue
- 6
- Pages
- 887-897
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2151315634
- Cited by
- 320
- References
- 22
- FWCI
- 10.84
- Type
- journal article
- Language
- en
- Open access
- green
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