The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis
Open accessAbstract
Focal adhesion kinase (FAK) is a critical mediator of signal transduction by integrins and growth factor receptors in a variety of cells including endothelial cells (ECs). Here, we describe EC-specific knockout of FAK using a Cre-loxP approach. In contrast to the total FAK knockout, deletion of FAK specifically in ECs did not affect early embryonic development including normal vasculogenesis. However, in late embryogenesis, FAK deletion in the ECs led to defective angiogenesis in the embryos, yolk sac, and placenta, impaired vasculature and associated hemorrhage, edema, and developmental delay, and late embryonic lethal phenotype. Histologically, ECs and blood vessels in the mutant embryos present a disorganized, detached, and apoptotic appearance. Consistent with these phenotypes, deletion of FAK in ECs isolated from the floxed FAK mice led to reduced tubulogenesis, cell survival, proliferation, and migration in vitro. Together, these results strongly suggest a role of FAK in angiogenesis and vascular development due to its essential function in the regulation of multiple EC activities.
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Cites
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Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
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Focal adhesion kinase: the first ten years
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Tie2-Cre Transgenic Mice: A New Model for Endothelial Cell-Lineage Analysis in Vivo
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Cbl-b regulates the CD28 dependence of T-cell activation
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Self-citationCary, L. A., Chang, J. F., & Guan, J.-L. (1996) · Journal of Cell Science · doi:10.1242/jcs.109.7.1787 · Cited by 596
Self-citationTargeted disruption of the three Rb-related genes leads to loss of G 1 control and immortalization
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Self-citationpp125FAK-Dependent Tyrosine Phosphorylation of Paxillin Creates a High-Affinity Binding Site for Crk
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Cited by
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Sulzmaier, F. J., Jean, C., & Schlaepfer, D. D. (2014) · Nature Reviews Cancer · doi:10.1038/nrc3792 · Cited by 1,381
Integrins in angiogenesis and lymphangiogenesis
Avraamides, C. J., Garmy-Susini, B., & Varner, J. A. (2008) · Nature Reviews Cancer · doi:10.1038/nrc2353 · Cited by 1,050
Focal adhesion kinase and its signaling pathways in cell migration and angiogenesis
Zhao, X., & Guan, J.-L. (2011) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.11.001 · Cited by 835
Self-citationHiratsuka, S., Goel, S., Kamoun, W. S., Maru, Y., Fukumura, D., Duda, D. G., & Jain, R. K. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100446108 · Cited by 196
Vimentin as an Integral Regulator of Cell Adhesion and Endothelial Sprouting
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Metadata
- Authors
- Shen, T.-L., Park, A. Y.-J., Alcaraz, A., Peng, X., Jang, I., Koni, P., Flavell, R. A., Gu, H., & Guan, J.-L.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-06-20
- Volume
- 169
- Issue
- 6
- Pages
- 941-952
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2142682878
- Cited by
- 304
- References
- 42
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Shen, T.-L., Park, A. Y.-J., Alcaraz, A., Peng, X., Jang, I., Koni, P., Flavell, R. A., Gu, H., & Guan, J.-L. (2005). Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200411155
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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