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Aurora A activates D-TACC–Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules
Open accessAbstract
Centrosomes are the dominant sites of microtubule (MT) assembly during mitosis in animal cells, but it is unclear how this is achieved. Transforming acidic coiled coil (TACC) proteins stabilize MTs during mitosis by recruiting Minispindles (Msps)/XMAP215 proteins to centrosomes. TACC proteins can be phosphorylated in vitro by Aurora A kinases, but the significance of this remains unclear. We show that Drosophila melanogaster TACC (D-TACC) is phosphorylated on Ser863 exclusively at centrosomes during mitosis in an Aurora A–dependent manner. In embryos expressing only a mutant form of D-TACC that cannot be phosphorylated on Ser863 (GFP-S863L), spindle MTs are partially destabilized, whereas astral MTs are dramatically destabilized. GFP-S863L is concentrated at centrosomes and recruits Msps there but cannot associate with the minus ends of MTs. We propose that the centrosomal phosphorylation of D-TACC on Ser863 allows D-TACC–Msps complexes to stabilize the minus ends of centrosome-associated MTs. This may explain why centrosomes are such dominant sites of MT assembly during mitosis.
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Citations
Cited by
Mechanisms of Asymmetric Stem Cell Division
Knoblich, J. A. (2008) · Cell · doi:10.1016/j.cell.2008.02.007 · Cited by 1,015
Basto, R., Lau, J., Vinogradova, T., Gardiol, A., Woods, C. G., Khodjakov, A., & Raff, J. W. (2006) · Cell · doi:10.1016/j.cell.2006.05.025 · Cited by 686
Self-citationRoles of Aurora Kinases in Mitosis and Tumorigenesis
Fu, J., Bian, M., Jiang, Q., & Zhang, C. (2007) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-06-0208 · Cited by 614
Aurora A kinase (AURKA) in normal and pathological cell division
Nikonova, A. S., Astsaturov, I., Serebriiskii, I. G., Dunbrack, R. L., & Golemis, E. A. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1073-7 · Cited by 448
Aurora-A: the maker and breaker of spindle poles
Barr, A. R., & Gergely, F. (2007) · Journal of Cell Science · doi:10.1242/jcs.013136 · Cited by 441
Metadata
- Authors
- Barros, T. P., Kinoshita, K., Hyman, A. A., & Raff, J. W.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-09-26
- Volume
- 170
- Issue
- 7
- Pages
- 1039-1046
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2162391239
- Cited by
- 165
- References
- 0
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Aurora A activates D-TACC–Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. https://app.cochat.ai/papers/10.1083/jcb.200504097
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- Barros, T. P., Kinoshita, K., Hyman, A. A., & Raff, J. W. (2005). Aurora A activates D-TACC–Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200504097
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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