The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state
Open accessAbstract
The tight junction defines epithelial organization. Structurally, the tight junction is comprised of transmembrane and membrane-associated proteins that are thought to assemble into stable complexes to determine function. In this study, we measure tight junction protein dynamics in live confluent Madin–Darby canine kidney monolayers using fluorescence recovery after photobleaching and related methods. Mathematical modeling shows that the majority of claudin-1 (76 ± 5%) is stably localized at the tight junction. In contrast, the majority of occludin (71 ± 3%) diffuses rapidly within the tight junction with a diffusion constant of 0.011 μm2s−1. Zonula occludens-1 molecules are also highly dynamic in this region, but, rather than diffusing within the plane of the membrane, 69 ± 5% exchange between membrane and intracellular pools in an energy-dependent manner. These data demonstrate that the tight junction undergoes constant remodeling and suggest that this dynamic behavior may contribute to tight junction assembly and regulation.
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Cites
JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA
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Occludin: a novel integral membrane protein localizing at tight junctions.
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Multifunctional strands in tight junctions
Tsukita, S., Furuse, M., & Itoh, M. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35067088 · Cited by 2,509
Furuse, M., Fujita, K., Hiiragi, T., Fujimoto, K., & Tsukita, S. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.7.1539 · Cited by 2,095
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A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
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Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
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Cited by
Klaassen, I., Van Noorden, C. J. F., & Schlingemann, R. O. (2013) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2013.02.001 · Cited by 722
Knowland, D., Arac, A., Sekiguchi, K. J., Hsu, M., Lutz, S. E., Perrino, J., Steinberg, G. K., Barres, B. A., Nimmerjahn, A., & Agalliu, D. (2014) · Neuron · doi:10.1016/j.neuron.2014.03.003 · Cited by 703
Landy, J., Ronde, E., English, N., Clark, S. K., Hart, A. L., Knight, S. C., Ciclitira, P. J., & Al-Hassi, H. O. (2016) · World Journal of Gastroenterology · doi:10.3748/wjg.v22.i11.3117 · Cited by 507
Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation
Garcia‐Hernandez, V., Quiros, M., & Nusrat, A. (2017) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.13360 · Cited by 445
Early events in cell adhesion and polarity during epithelial-mesenchymal transition
Huang, R. Y.-J., Guilford, P., & Thiery, J. P. (2012) · Journal of Cell Science · doi:10.1242/jcs.099697 · Cited by 369
Metadata
- Authors
- Shen, L., Weber, C. R., & Turner, J. R.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2008-05-12
- Volume
- 181
- Issue
- 4
- Pages
- 683-695
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2157593452
- Cited by
- 359
- References
- 68
- FWCI
- 14.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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