The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation
Open accessAbstract
Focal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II–mediated cytoskeletal tension in a process known as FA maturation. To understand tension-mediated FA maturation, we sought to identify proteins that are recruited to FAs in a myosin II–dependent manner and to examine the mechanism for their myosin II–sensitive FA association. We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent. Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin. We show that phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. These results reveal an important role for paxillin in adhesion mechanosensing via myosin II–mediated FAK phosphorylation of paxillin that promotes vinculin FA recruitment to reinforce the cytoskeletal ECM linkage and drive FA maturation.
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Cites
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CELLULAR FUNCTIONS REGULATED BY SRC FAMILY KINASES
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“Tension on FA proteins could drive localized unfolding or conformational changes that unmask binding sites for cytoplasmic proteins (Vogel and Sheetz, 2006).”
Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
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Stretching Single Talin Rod Molecules Activates Vinculin Binding
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Focal Contacts as Mechanosensors
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Cited by
Mechanotransduction and extracellular matrix homeostasis
Humphrey, J. D., Dufresne, E. R., & Schwartz, M. A. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3896 · Cited by 2,124
Integrins as biomechanical sensors of the microenvironment
Kechagia, J. Z., Ivaska, J., & Roca-Cusachs, P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0134-2 · Cited by 1,311
Materials as stem cell regulators
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Integrins and Extracellular Matrix in Mechanotransduction
Schwartz, M. A. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005066 · Cited by 662
le Duc, Q., Shi, Q., Blonk, I., Sonnenberg, A., Wang, N., Leckband, D., & de Rooij, J. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201001149 · Cited by 652
Metadata
- Authors
- Pasapera, A. M., Schneider, I. C., Rericha, E., Schlaepfer, D. D., & Waterman, C. M.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-03-22
- Volume
- 188
- Issue
- 6
- Pages
- 877-890
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2145392559
- Cited by
- 572
- References
- 62
- FWCI
- 15.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Pasapera, A. M., Schneider, I. C., Rericha, E., Schlaepfer, D. D., & Waterman, C. M. (2010). Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. Journal of Cell Biology. https://doi.org/10.1083/jcb.200906012
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