The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Automated microscopy for high-content RNAi screening
Open accessAbstract
Fluorescence microscopy is one of the most powerful tools to investigate complex cellular processes such as cell division, cell motility, or intracellular trafficking. The availability of RNA interference (RNAi) technology and automated microscopy has opened the possibility to perform cellular imaging in functional genomics and other large-scale applications. Although imaging often dramatically increases the content of a screening assay, it poses new challenges to achieve accurate quantitative annotation and therefore needs to be carefully adjusted to the specific needs of individual screening applications. In this review, we discuss principles of assay design, large-scale RNAi, microscope automation, and computational data analysis. We highlight strategies for imaging-based RNAi screening adapted to different library and assay designs.
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Cell death assays for drug discovery
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SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
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Metadata
- Authors
- Conrad, C., & Gerlich, D. W.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-02-22
- Volume
- 188
- Issue
- 4
- Pages
- 453-461
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2056022845
- Cited by
- 168
- References
- 8
- FWCI
- 12.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
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