The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Dual recognition of CENP-A nucleosomes is required for centromere assembly
Open accessAbstract
Centromeres contain specialized nucleosomes in which histone H3 is replaced by the histone variant centromere protein A (CENP-A). CENP-A nucleosomes are thought to act as an epigenetic mark that specifies centromere identity. We previously identified CENP-N as a CENP-A nucleosome-specific binding protein. Here, we show that CENP-C also binds directly and specifically to CENP-A nucleosomes. Nucleosome binding by CENP-C required the extreme C terminus of CENP-A and did not compete with CENP-N binding, which suggests that CENP-C and CENP-N recognize distinct structural elements of CENP-A nucleosomes. A mutation that disrupted CENP-C binding to CENP-A nucleosomes in vitro caused defects in CENP-C targeting to centromeres. Moreover, depletion of CENP-C with siRNA resulted in the mislocalization of all other nonhistone CENPs examined, including CENP-K, CENP-H, CENP-I, and CENP-T, and led to a partial reduction in centromeric CENP-A. We propose that CENP-C binds directly to CENP-A chromatin and, together with CENP-N, provides the foundation upon which other centromere and kinetochore proteins are assembled.
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Cites
Boveri revisited: chromosomal instability, aneuploidy and tumorigenesis
Holland, A. J., & Cleveland, D. W. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2718 · Cited by 859
Cited by
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome
Yatskevich, S., Muir, K. W., Bellini, D., Zhang, Z., Yang, J., Tischer, T., Predin, M., Dendooven, T., McLaughlin, S. H., & Barford, D. (2022) · Science · doi:10.1126/science.abn3810 · Cited by 120
Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
Dendooven, T., Zhang, Z., Yang, J., McLaughlin, S. H., Schwab, J., Scheres, S. H. W., Yatskevich, S., & Barford, D. (2023) · Science Advances · doi:10.1126/sciadv.adg7480 · Cited by 58
Insects evolved a monomeric histone-fold domain in the CENP-T protein family
Sankaranarayanan, S. R., Ulmer, J., Mørch, A., Ali-Ahmad, A., Sekulić, N., & Drinnenberg, I. A. (2025) · EMBO Reports · doi:10.1038/s44319-025-00603-5 · Cited by 2
Metadata
- Authors
- Carroll, C. W., Milks, K. J., & Straight, A. F.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-06-21
- Volume
- 189
- Issue
- 7
- Pages
- 1143-1155
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2075763595
- Cited by
- 345
- References
- 1
- FWCI
- 10.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
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