The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells
Open accessAbstract
LIM kinases 1 and 2 (LIMK1/2) are centrally positioned regulators of actin cytoskeleton dynamics. Using siRNA-mediated knockdown or a novel small molecule inhibitor, we show LIMK is required for path generation by leading tumor cells and nontumor stromal cells during collective tumor cell invasion. LIMK inhibition lowers cofilin phosphorylation, F-actin levels, serum response factor transcriptional activity and collagen contraction, and reduces invasion in three-dimensional invasion assays. Although motility was unaffected, LIMK inhibition impairs matrix protein degradation and invadopodia formation associated with significantly faster recovery times in FRAP assays indicative of reduced F-actin stability. When LIMK is knocked down in MDA-MB-231 cells, they lose the ability to lead strands of collectively invading cells. Similarly, when LIMK activity is blocked in cancer-associated fibroblasts, they are unable to lead the collective invasion of squamous carcinoma cells in an organotypic skin model. These results show that LIMK is required for matrix remodeling activities for path generation by leading cells in collective invasion.
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Cites
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Gaggioli, C., Hooper, S., Hidalgo-Carcedo, C., Grosse, R., Marshall, J. F., Harrington, K., & Sahai, E. (2007) · Nature Cell Biology · doi:10.1038/ncb1658 · Cited by 1,589 · cited 6×
“It is also possible for noninvasive cells to become invasive by following tracks laid down by other path-generating cells; in fact path generation may occur temporally in advance of invasion (Gaggioli et al., 2007).”
BackgroundMethodInfluential
Cited by
Ridley, A. J. (2011) · Cell · doi:10.1016/j.cell.2011.06.010 · Cited by 956
Functions of cofilin in cell locomotion and invasion
Bravo-Cordero, J. J., Magalhaes, M. A. O., Eddy, R. J., Hodgson, L., & Condeelis, J. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3609 · Cited by 461
Cancer-Associated Fibroblasts as Another Polarized Cell Type of the Tumor Microenvironment
Augsten, M. (2014) · Frontiers in Oncology · doi:10.3389/fonc.2014.00062 · Cited by 427
Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation
Mizuno, K. (2013) · Cellular Signalling · doi:10.1016/j.cellsig.2012.11.001 · Cited by 396
Cell Migration and Invasion Assays as Tools for Drug Discovery
Hulkower, K. I., & Herber, R. L. (2011) · Pharmaceutics · doi:10.3390/pharmaceutics3010107 · Cited by 395
Metadata
- Authors
- Scott, R. W., Hooper, S., Crighton, D., Li, A., König, I., Munro, J., Trivier, E., Wickman, G., Morin, P., Croft, D. R., Dawson, J., Machesky, L., Anderson, K. I., Sahai, E. A., & Olson, M. F.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-09-27
- Volume
- 191
- Issue
- 1
- Pages
- 169-185
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2135174117
- Cited by
- 175
- References
- 2
- FWCI
- 7.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Scott, R. W., Hooper, S., Crighton, D., Li, A., König, I., Munro, J., Trivier, E., Wickman, G., Morin, P., Croft, D. R., Dawson, J., Machesky, L., Anderson, K. I., Sahai, E. A., & Olson, M. F. (2010). LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells. Journal of Cell Biology. https://doi.org/10.1083/jcb.201002041
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