The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3
Open accessAbstract
The molecules that regulate the apoptosis cascade are also involved in differentiation and syncytial fusion in skeletal muscle. MyoD is a myogenic transcription factor that plays essential roles in muscle differentiation. We noticed that MyoD−/− myoblasts display remarkable resistance to apoptosis by down-regulation of miR-1 (microRNA-1) and miR-206 and by up-regulation of Pax3. This resulted in transcriptional activation of antiapoptotic factors Bcl-2 and Bcl-xL. Forced MyoD expression induces up-regulation of miR-1 and miR-206 and down-regulation of Pax3, Bcl-2, and Bcl-xL along with increased apoptosis in MyoD−/− myoblasts. In contrast, MyoD gene knockdown increases cell survival of wild-type myoblasts. The 3′ untranslated region of Pax3 mRNA contains two conserved miR-1/miR-206–binding sites, which are required for targeting of these microRNAs (miRNAs). Therefore, these data suggest that MyoD not only regulates terminal differentiation but also apoptosis through miRNA-mediated down-regulation of Pax3. Finally, MyoD, miR-1, and miR-206 are all down-regulated in quiescent satellite cells, which may be required for maintenance of muscle stem cells.
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Cites
Asymmetric self-renewal and commitment of satellite stem cells in muscle
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“Lastly, double-positive cells for integrin-7 Alexa Fluor 488 and integrin-1 APC were sorted to enrich for QSCs (Kuang et al., 2007).”
InfluentialCollins, C. A., Olsen, I., Zammit, P. S., Heslop, L., Petrie, A., Partridge, T. A., & Morgan, J. E. (2005) · Cell · doi:10.1016/j.cell.2005.05.010 · Cited by 1,445
Self-renewal and expansion of single transplanted muscle stem cells
Sacco, A., Doyonnas, R., Kraft, P., Vitorovic, S., & Blau, H. M. (2008) · Nature · doi:10.1038/nature07384 · Cited by 901
MyoD is required for myogenic stem cell function in adult skeletal muscle
Megeney, L. A., Kablar, B., Garrett, K., Anderson, J. E., & Rudnicki, M. A. (1996) · Genes & Development · doi:10.1101/gad.10.10.1173 · Cited by 724
Molecular Signature of Quiescent Satellite Cells in Adult Skeletal Muscle
Fukada, S.-I., Uezumi, A., Ikemoto, M., Masuda, S., Segawa, M., Tanimura, N., Yamamoto, H., Miyagoe-Suzuki, Y., & Takeda, S. (2007) · Stem Cells · doi:10.1634/stemcells.2007-0019 · Cited by 506
Highly Efficient, Functional Engraftment of Skeletal Muscle Stem Cells in Dystrophic Muscles
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CTCF-binding sites flank CTG/CAG repeats and form a methylation-sensitive insulator at the DM1 locus
Filippova, G. N., Thienes, C. P., Penn, B. H., Cho, D. H., Hu, Y. J., Moore, J. M., Klesert, T. R., Lobanenkov, V. V., & Tapscott, S. J. (2001) · Nature Genetics · doi:10.1038/ng570 · Cited by 332
Cited by
Satellite Cells and the Muscle Stem Cell Niche
Yin, H., Price, F., & Rudnicki, M. A. (2013) · Physiological Reviews · doi:10.1152/physrev.00043.2011 · Cited by 2,166
Building Muscle: Molecular Regulation of Myogenesis
Bentzinger, C. F., Wang, Y. X., & Rudnicki, M. A. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a008342 · Cited by 1,195
Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis
Braun, T., & Gautel, M. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3118 · Cited by 713
Muscle-specific microRNAs in skeletal muscle development
Horak, M., Novak, J., & Bienertova-Vasku, J. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2015.12.013 · Cited by 478
Misregulation of miR-1 processing is associated with heart defects in myotonic dystrophy
Rau, F., Freyermuth, F., Fugier, C., Villemin, J.-P., Fischer, M.-C., Jost, B., Dembele, D., Gourdon, G., Nicole, A., Duboc, D., et al. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2067 · Cited by 280
Metadata
- Authors
- Hirai, H., Verma, M., Watanabe, S., Tastad, C., Asakura, Y., & Asakura, A.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-10-18
- Volume
- 191
- Issue
- 2
- Pages
- 347-365
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2046548473
- Cited by
- 179
- References
- 7
- FWCI
- 6.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hirai, H., Verma, M., Watanabe, S., Tastad, C., Asakura, Y., & Asakura, A. (2010). MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3. Journal of Cell Biology. https://doi.org/10.1083/jcb.201006025
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