MyoD is required for myogenic stem cell function in adult skeletal muscle
Open accessAbstract
To investigate the function of MyoD in adult skeletal muscle, we interbred MyoD mutant mice with mdx mice, a model for Duchenne and Becker muscular dystrophy. Mice lacking both MyoD and dystrophin displayed a marked increase in severity of myopathy leading to premature death, suggesting a role for MyoD in muscle regeneration. Examination of MyoD mutant muscle revealed elevated numbers of myogenic cells; however, myoblasts derived from these cells displayed normal differentiation potential in vitro. Following injury, MyoD mutant muscle was severely deficient in regenerative ability, and we observed a striking reduction in the in vivo proliferation of myogenic cells during regeneration. Therefore, we propose that the failure of MyoD-deficient muscle to regenerate efficiently is not caused by a reduction in numbers of satellite cells, the stem cells of adult skeletal muscle, but results from an increased propensity for stem-cell self-renewal rather than progression through the myogenic program.
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Murine muscle stem cells (MuSCs) experience a transition from quiescence to activation that is required for regeneration.
“Murine muscle stem cells (MuSCs) experience a transition from quiescence to activation that is required for regeneration, but it remains unknown if the trajectory and dynamics of activation change with age.”
From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Megeney et al., 1996
Impaired stem cell activation impairs regenerative potential in multiple tissues.
“Impaired stem cell activation has been shown to impair regenerative potential in multiple tissues (Megeney et al., 1996; Zou et al., 2018).”
From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Megeney et al., 1996
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- Authors
- Megeney, L. A., Kablar, B., Garrett, K., Anderson, J. E., & Rudnicki, M. A.
- Affiliations
- McMaster University (CA)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1996-05-15
- Volume
- 10
- Issue
- 10
- Pages
- 1173-1183
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2091101448
- Cited by
- 724
- References
- 46
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 9 · 2023: 20 · 2022: 18 · 2021: 18 · 2020: 30 · 2019: 26 · 2018: 26 · 2017: 25 · 2016: 30 · 2015: 28 · 2014: 34 · 2013: 30 · 2012: 27
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Muscular Dystrophy Association
- Manitoba Health Research Council
- Medical Research Council Canada
- Medical Research Council
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- MyoD, myogenic stem cells, satellite cells, skeletal muscle regeneration, Duchenne muscular dystrophy, Becker muscular dystrophy, mdx mice, dystrophin, myogenesis, stem cell self-renewal, muscle damage
- MeSH terms
- Animals, Cell Division, Cells, Cultured, Regeneration, Stem Cells, Dystrophin, Gene Deletion, MyoD Protein, Mice, Inbred mdx, Muscle, Skeletal, Mice
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