The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision
Open accessAbstract
Correlative electron and fluorescence microscopy has the potential to elucidate the ultrastructural details of dynamic and rare cellular events, but has been limited by low precision and sensitivity. Here we present a method for direct mapping of signals originating from ∼20 fluorescent protein molecules to 3D electron tomograms with a precision of less than 100 nm. We demonstrate that this method can be used to identify individual HIV particles bound to mammalian cell surfaces. We also apply the method to image microtubule end structures bound to mal3p in fission yeast, and demonstrate that growing microtubule plus-ends are flared in vivo. We localize Rvs167 to endocytic sites in budding yeast, and show that scission takes place halfway through a 10-s time period during which amphiphysins are bound to the vesicle neck. This new technique opens the door for direct correlation of fluorescence and electron microscopy to visualize cellular processes at the ultrastructural scale.
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Cites
Tracking the ends: a dynamic protein network controls the fate of microtubule tips
Akhmanova, A., & Steinmetz, M. O. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2369 · Cited by 1,047
A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2005) · Cell · doi:10.1016/j.cell.2005.09.024 · Cited by 726 · cited 3×
“The dynamic organization of the endocytic machinery in budding yeast has been elucidated using live-cell imaging of fluorescent protein–labeled endocytic proteins (Kaksonen et al., 2003, 2005).”
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Cited by
Correlated light and electron microscopy: ultrastructure lights up!
de Boer, P., Hoogenboom, J. P., & Giepmans, B. N. G. (2015) · Nature Methods · doi:10.1038/nmeth.3400 · Cited by 526
Snijder, E. J., Limpens, R. W. A. L., de Wilde, A. H., de Jong, A. W. M., Zevenhoven-Dobbe, J. C., Maier, H. J., Faas, F. F. G. A., Koster, A. J., & Bárcena, M. (2020) · PLOS Biology · doi:10.1371/journal.pbio.3000715 · Cited by 477
Roles for actin assembly in endocytosis
Mooren, O. L., Galletta, B. J., & Cooper, J. A. (2012) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060910-094416 · Cited by 394
Cone-shaped HIV-1 capsids are transported through intact nuclear pores
Zila, V., Margiotta, E., Turoňová, B., Müller, T. G., Zimmerli, C. E., Mattei, S., Allegretti, M., Börner, K., Rada, J., Müller, B., et al. (2021) · Cell · doi:10.1016/j.cell.2021.01.025 · Cited by 339
Plasma Membrane Reshaping during Endocytosis Is Revealed by Time-Resolved Electron Tomography
Kukulski, W., Schorb, M., Kaksonen, M., & Briggs, J. A. G. (2012) · Cell · doi:10.1016/j.cell.2012.05.046 · Cited by 332
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Metadata
- Authors
- Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M., & Briggs, J. A. G.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2011-01-03
- Volume
- 192
- Issue
- 1
- Pages
- 111-119
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2145494795
- Cited by
- 467
- References
- 2
- FWCI
- 21.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
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