The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets
Open accessAbstract
Lipins are phosphatidate phosphatases that generate diacylglycerol (DAG). In this study, we report that yeast lipin, Pah1p, controls the formation of cytosolic lipid droplets. Disruption of PAH1 resulted in a 63% decrease in droplet number, although total neutral lipid levels did not change. This was accompanied by an accumulation of neutral lipids in the endoplasmic reticulum (ER). The droplet biogenesis defect was not a result of alterations in neutral lipid ratios. No droplets were visible in the absence of both PAH1 and steryl acyltransferases when grown in glucose medium, even though the strain produces as much triacylglycerol as wild type. The requirement of PAH1 for normal droplet formation can be bypassed by a knockout of DGK1. Nem1p, the activator of Pah1p, localizes to a single punctum per cell on the ER that is usually next to a droplet, suggesting that it is a site of droplet assembly. Overall, this study provides strong evidence that DAG generated by Pah1p is important for droplet biogenesis.
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Cites
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Adipocyte differentiation from the inside out
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Cited by
Dynamics and functions of lipid droplets
Olzmann, J. A., & Carvalho, P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0085-z · Cited by 2,740
Lipid droplet biogenesis and functions in health and disease
Zadoorian, A., Du, X., & Yang, H. (2023) · Nature Reviews Endocrinology · doi:10.1038/s41574-023-00845-0 · Cited by 617
Biogenesis of the multifunctional lipid droplet: Lipids, proteins, and sites
Pol, A., Gross, S. P., & Parton, R. G. (2014) · Journal of Cell Biology · doi:10.1083/jcb.201311051 · Cited by 466
Oleaginous yeasts for biodiesel: Current and future trends in biology and production
Sitepu, I. R., Garay, L. A., Sestric, R., Levin, D., Block, D. E., German, J. B., & Boundy-Mills, K. L. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.08.003 · Cited by 465
Wilfling, F., Haas, J. T., Walther, T. C., & Jr, R. V. F. (2014) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2014.03.008 · Cited by 408
Metadata
- Authors
- Adeyo, O., Horn, P. J., Lee, S., Binns, D. D., Chandrahas, A., Chapman, K. D., & Goodman, J. M.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2011-03-21
- Volume
- 192
- Issue
- 6
- Pages
- 1043-1055
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2116613524
- Cited by
- 300
- References
- 66
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Adeyo, O., Horn, P. J., Lee, S., Binns, D. D., Chandrahas, A., Chapman, K. D., & Goodman, J. M. (2011). The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets. Journal of Cell Biology. https://doi.org/10.1083/jcb.201010111
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