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Cited by 182

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex

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Kawai, Shinji, Amano, Atsuo·2012·Journal of Cell Biology
DOI 10.1083/jcb.201110008
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Abstract

MicroRNAs (miRNAs) are noncoding RNAs that function as key posttranscriptional regulators of gene expression. miRNA maturation is controlled by the DROSHA microprocessor complex. However, the detailed mechanism of miRNA biogenesis remains unclear. We show that the tumor suppressor breast cancer 1 (BRCA1) accelerates the processing of miRNA primary transcripts. BRCA1 increased the expressions of both precursor and mature forms of let-7a-1, miR-16-1, miR-145, and miR-34a. In addition, this tumor suppressor was shown to be directly associated with DROSHA and DDX5 of the DROSHA microprocessor complex, and it interacted with Smad3, p53, and DHX9 RNA helicase. We also found that BRCA1 recognizes the RNA secondary structure and directly binds with primary transcripts of miRNAs via a DNA-binding domain. Together, these results suggest that BRCA1 regulates miRNA biogenesis via the DROSHA microprocessor complex and Smad3/p53/DHX9. Our findings also indicate novel functions of BRCA1 in miRNA biogenesis, which may be linked to its tumor suppressor mechanism and maintenance of genomic stability.

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Research works printed but not linked: 28

  1. Anderson, 1998, BRCA1 protein is linked to the RNA polymerase II holoenzyme complex via RNA helicase A, Nat. Genet., 19, 254, 10.1038/930 · DOI · Paper page
  2. Calin, 2004, Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers, Proc. Natl. Acad. Sci. USA., 101, 2999, 10.1073/pnas.0307323101 · DOI · Paper page
  3. Cimmino, 2005, miR-15 and miR-16 induce apoptosis by targeting BCL2, Proc. Natl. Acad. Sci. USA., 102, 13944, 10.1073/pnas.0506654102 · DOI · Paper page
  4. Davis, 2008, SMAD proteins control DROSHA-mediated microRNA maturation, Nature., 454, 56, 10.1038/nature07086 · DOI · Paper page
  5. Dubrovska, 2005, TGFbeta1/Smad3 counteracts BRCA1-dependent repair of DNA damage, Oncogene., 24, 2289, 10.1038/sj.onc.1208443 · DOI
  6. Fukuda, 2007, DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs, Nat. Cell Biol., 9, 604, 10.1038/ncb1577 · DOI · Paper page
  7. Fuller-Pace, 2006, DExD/H box RNA helicases: multifunctional proteins with important roles in transcriptional regulation, Nucleic Acids Res., 34, 4206, 10.1093/nar/gkl460 · DOI · Paper page
  8. Huen, 2010, BRCA1 and its toolbox for the maintenance of genome integrity, Nat. Rev. Mol. Cell Biol., 11, 138, 10.1038/nrm2831 · DOI · Paper page
  9. Jhanwar-Uniyal, 2003, BRCA1 in cancer, cell cycle and genomic stability, Front. Biosci., 8, s1107, 10.2741/1131 · DOI
  10. Johnson, 2005, RAS is regulated by the let-7 microRNA family, Cell., 120, 635, 10.1016/j.cell.2005.01.014 · DOI · Paper page
  11. Kumar, 2007, Impaired microRNA processing enhances cellular transformation and tumorigenesis, Nat. Genet., 39, 673, 10.1038/ng2003 · DOI · Paper page
  12. Lee, 2003, The nuclear RNase III Drosha initiates microRNA processing, Nature., 425, 415, 10.1038/nature01957 · DOI · Paper page
  13. Lee, 2007, Expression profiling identifies microRNA signature in pancreatic cancer, Int. J. Cancer., 120, 1046, 10.1002/ijc.22394 · DOI · Paper page
  14. Lee, 2009, MicroRNA profiling of BRCA1/2 mutation-carrying and non-mutation-carrying high-grade serous carcinomas of ovary, PLoS ONE., 4, e7314, 10.1371/journal.pone.0007314 · DOI
  15. Lu, 2005, MicroRNA expression profiles classify human cancers, Nature., 435, 834, 10.1038/nature03702 · DOI · Paper page
  16. Marson, 2008, Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells, Cell., 134, 521, 10.1016/j.cell.2008.07.020 · DOI · Paper page
  17. Osaki, 2008, MicroRNAs as biomarkers and therapeutic drugs in human cancer, Biomarkers., 13, 658, 10.1080/13547500802646572 · DOI
  18. Schlegel, 2003, Overexpression of a protein fragment of RNA helicase A causes inhibition of endogenous BRCA1 function and defects in ploidy and cytokinesis in mammary epithelial cells, Oncogene., 22, 983, 10.1038/sj.onc.1206195 · DOI
  19. Shen, 1998, A targeted disruption of the murine Brca1 gene causes gamma-irradiation hypersensitivity and genetic instability, Oncogene., 17, 3115, 10.1038/sj.onc.1202243 · DOI · Paper page
  20. Slaby, 2007, Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer, Oncology., 72, 397, 10.1159/000113489 · DOI · Paper page
  21. Suzuki, 2009, Modulation of microRNA processing by p53, Nature., 460, 529, 10.1038/nature08199 · DOI · Paper page
  22. Szafranska, 2007, MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma, Oncogene., 26, 4442, 10.1038/sj.onc.1210228 · DOI · Paper page
  23. Takahashi, 2006, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell., 126, 663, 10.1016/j.cell.2006.07.024 · DOI · Paper page
  24. Venkitaraman, 2002, Cancer susceptibility and the functions of BRCA1 and BRCA2, Cell., 108, 171, 10.1016/S0092-8674(02)00615-3 · DOI · Paper page
  25. Wilson, 2007, Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis, BMC Genomics., 8, 419, 10.1186/1471-2164-8-419 · DOI
  26. Xu, 2009, MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells, Cell., 137, 647, 10.1016/j.cell.2009.02.038 · DOI · Paper page
  27. Yamagata, 2009, Maturation of microRNA is hormonally regulated by a nuclear receptor, Mol. Cell., 36, 340, 10.1016/j.molcel.2009.08.017 · DOI · Paper page
  28. Zhang, 1998, BRCA1 physically associates with p53 and stimulates its transcriptional activity, Oncogene., 16, 1713, 10.1038/sj.onc.1201932 · DOI · Paper page

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Metadata

Authors
Kawai, S., & Amano, A.
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2012-04-09
Volume
197
Issue
2
Pages
201-208
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.201110008
OpenAlex
W2116005916
Cited by
182
References
2
FWCI
6.41
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex. https://app.cochat.ai/papers/10.1083/jcb.201110008
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Kawai, S., & Amano, A. (2012). BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex. Journal of Cell Biology. https://doi.org/10.1083/jcb.201110008

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