The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy
Open accessAbstract
The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), of which ZP2 is proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage is associated with exocytosis of cortical granules that are peripherally located subcellular organelles unique to ovulated eggs. Based on the cleavage site of ZP2, ovastacin was selected as a candidate protease. Encoded by the single-copy Astl gene, ovastacin is an oocyte-specific member of the astacin family of metalloendoproteases. Using specific antiserum, ovastacin was detected in cortical granules before, but not after, fertilization. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease. Female mice lacking ovastacin did not cleave ZP2 after fertilization, and mouse sperm bound as well to Astl-null two-cell embryos as they did to normal eggs. Ovastacin is a pioneer component of mouse cortical granules and plays a definitive role in the postfertilization block to sperm binding that ensures monospermic fertilization and successful development.
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1 retracted
Identification and Characterization of Human and Mouse Ovastacin
Quesada, V., Sánchez, L. M., Álvarez, J., & López-Otı́n, C. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m401588200 · Cited by 93
RetractedSperm transport in the female reproductive tract
Suarez, S. S., & Pacey, A. A. (2006) · Human Reproduction Update · doi:10.1093/humupd/dmi047 · Cited by 1,113
A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
Liu, P., Jenkins, N. A., & Copeland, N. G. (2003) · Genome Research · doi:10.1101/gr.749203 · Cited by 1,013
Bleil, J. D., & Wassarman, P. M. (1980) · Developmental Biology · doi:10.1016/0012-1606(80)90371-1 · Cited by 627
Maternal control of early mouse development
Li, L., Zheng, P., & Dean, J. (2010) · Development · doi:10.1242/dev.039487 · Cited by 481
Self-citationEgg-to-Embryo Transition Is Driven by Differential Responses to Ca2+ Oscillation Number
Ducibella, T., Huneau, D., Angelichio, E., Xu, Z., Schultz, R. M., Kopf, G. S., Fissore, R., Madoux, S., & Ozil, J.-P. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0788 · Cited by 417
The astacin family of metalloendopeptidases
Bond, J. S., & Beynon, R. J. (1995) · Protein Science · doi:10.1002/pro.5560040701 · Cited by 408
Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development
Rankin, T. L., O’Brien, M. J., Lee, E., Wigglesworth, K., Eppig, J. J., & Dean, J. (2001) · Development · doi:10.1242/dev.128.7.1119 · Cited by 264
Self-citationBleil, J. D., Beall, C. F., & Wassarman, P. M. (1981) · Developmental Biology · doi:10.1016/0012-1606(81)90329-8 · Cited by 211
Gamete Recognition in Mice Depends on the Cleavage Status of an Egg’s Zona Pellucida Protein
Gahlay, G., Gauthier, L., Baibakov, B., Epifano, O., & Dean, J. (2010) · Science · doi:10.1126/science.1188178 · Cited by 186
Self-citation
Cited by
Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice
Nozawa, K., Satouh, Y., Fujimoto, T., Oji, A., & Ikawa, M. (2018) · Scientific Reports · doi:10.1038/s41598-018-19497-6 · Cited by 140
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat
Nishio, S., Emori, C., Wiseman, B., Fahrenkamp, D., Dioguardi, E., Zamora-Caballero, S., Bokhove, M., Han, L., Stsiapanava, A., Algarra, B., et al. (2024) · Cell · doi:10.1016/j.cell.2024.02.013 · Cited by 54
Metadata
- Authors
- Burkart, A. D., Xiong, B., Baibakov, B., Jiménez-Movilla, M., & Dean, J.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-04-02
- Volume
- 197
- Issue
- 1
- Pages
- 37-44
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2026486120
- Cited by
- 306
- References
- 26
- FWCI
- 14.47
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. https://app.cochat.ai/papers/10.1083/jcb.201112094
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- Burkart, A. D., Xiong, B., Baibakov, B., Jiménez-Movilla, M., & Dean, J. (2012). Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. Journal of Cell Biology. https://doi.org/10.1083/jcb.201112094
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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