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Cited by 299Angiogenesis and VEGF in CancerCell Adhesion Molecules ResearchCellular Mechanics and Interactions

Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex

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Coon, Brian G., Baeyens, Nicolas, Han, Jinah, Budatha, Madhusudhan, Ross, Tyler D., Fang, Jennifer S., Yun, Sanguk, Thomas, Jeon-Leon +1 more·2015·Journal of Cell Biology
DOI 10.1083/jcb.201408103PMC PMC4384728
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Abstract

Endothelial responses to fluid shear stress are essential for vascular development and physiology, and determine the formation of atherosclerotic plaques at regions of disturbed flow. Previous work identified VE-cadherin as an essential component, along with PECAM-1 and VEGFR2, of a complex that mediates flow signaling. However, VE-cadherin’s precise role is poorly understood. We now show that the transmembrane domain of VE-cadherin mediates an essential adapter function by binding directly to the transmembrane domain of VEGFR2, as well as VEGFR3, which we now identify as another component of the junctional mechanosensory complex. VEGFR2 and VEGFR3 signal redundantly downstream of VE-cadherin. Furthermore, VEGFR3 expression is observed in the aortic endothelium, where it contributes to flow responses in vivo. In summary, this study identifies a novel adapter function for VE-cadherin mediated by transmembrane domain association with VEGFRs.

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Authors
Coon, B. G., Baeyens, N., Han, J., Budatha, M., Ross, T. D., Fang, J. S., Yun, S., Thomas, J.-L., & Schwartz, M. A.
Affiliations
  • Yale University (US)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Schwartz, Martin A.
ORCID
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2015-03-23
Volume
208
Issue
7
Pages
975-986
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.201408103
PMC
PMC4384728
OpenAlex
W2161020445
Cited by
299
References
1
Citations by year
2026: 16 · 2025: 17 · 2024: 26 · 2023: 21 · 2022: 29 · 2021: 32 · 2020: 36 · 2019: 29 · 2018: 24 · 2017: 29 · 2016: 29 · 2015: 11
FWCI
13.45
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Research Explorer (The University of Manchester) (publishedVersion, other-oa)
  • Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion, other-oa)
Funders
  • National Institutes of Health (UL1TR000142)
  • National Heart, Lung, and Blood Institute (R01HL075092, T32HL007950)
  • National Center for Advancing Translational Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
VE-cadherin, VEGFR2, VEGFR-3, fluid shear stress, endothelial junctions, CD31, aortic endothelium, atherosclerosis, flow disturbance
MeSH terms
Cadherin 5, Animals, Cell Movement, Cells, Cultured, Endothelium, Vascular, Humans, Mice, Inbred C57BL, Stress, Physiological, Stress, Mechanical, Antigens, CD, Cadherins, Protein Structure, Tertiary, Neovascularization, Physiologic, Platelet Endothelial Cell Adhesion Molecule-1, RNA Interference, RNA, Small Interfering, Vascular Endothelial Growth Factor Receptor-2, Vascular Endothelial Growth Factor Receptor-3, Mechanotransduction, Cellular, Mice, HEK293 Cells, Plaque, Atherosclerotic, Human Umbilical Vein Endothelial Cells

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