The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization
Open accessAbstract
Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure. In cells lacking the RNA binding protein Fus, paraspeckle spheroids are disassembled into smaller particles containing Neat1, which are diffusely distributed in the nucleoplasm. Sequencing analysis of RNAs purified from paraspeckles revealed that AG-rich transcripts associate with Neat1, which are distributed along the shell of the paraspeckle spheroids. We propose that paraspeckles sequester core components inside the spheroids, whereas the outer surface associates with other components in the nucleoplasm to fulfill their function.
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Self-citationLong pre-mRNA depletion and RNA missplicing contribute to neuronal vulnerability from loss of TDP-43
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The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3
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Self-citationPhase separation and neurodegenerative diseases: a disturbance in the force
Zbinden, A., Pérez-Berlanga, M., De Rossi, P., & Polymenidou, M. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.09.014 · Cited by 500
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Martin, E. W., & Mittag, T. (2018) · Biochemistry · doi:10.1021/acs.biochem.8b00008 · Cited by 389
Modulation of RNA Condensation by the DEAD-Box Protein eIF4A
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Metadata
- Authors
- West, J. A., Mito, M., Kurosaka, S., Takumi, T., Tanegashima, C., Chujo, T., Yanaka, K., Kingston, R. E., Hirose, T., Bond, C., Fox, A., & Nakagawa, S.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2016-09-19
- Volume
- 214
- Issue
- 7
- Pages
- 817-830
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2522982067
- Cited by
- 328
- References
- 46
- FWCI
- 12.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- West, J. A., Mito, M., Kurosaka, S., Takumi, T., Tanegashima, C., Chujo, T., Yanaka, K., Kingston, R. E., Hirose, T., Bond, C., Fox, A., & Nakagawa, S. (2016). Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization. Journal of Cell Biology. https://doi.org/10.1083/jcb.201601071
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