An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles
Open accessAbstract
NEAT1 RNA, a highly abundant 4 kb ncRNA, is retained in nuclei in approximately 10 to 20 large foci that we show are completely coincident with paraspeckles, nuclear domains implicated in mRNA nuclear retention. Depletion of NEAT1 RNA via RNAi eradicates paraspeckles, suggesting that it controls sequestration of the paraspeckle proteins PSP1 and p54, factors linked to A-I editing. Unlike overexpression of PSP1, NEAT1 overexpression increases paraspeckle number, and paraspeckles emanate exclusively from the NEAT1 transcription site. The PSP-1 RNA binding domain is required for its colocalization with NEAT1 RNA in paraspeckles, and biochemical analyses support that NEAT1 RNA binds with paraspeckle proteins. Unlike other nuclear-retained RNAs, NEAT1 RNA is not A-I edited, consistent with a structural role in paraspeckles. Collectively, results demonstrate that NEAT1 functions as an essential structural determinant of paraspeckles, providing a precedent for a ncRNA as the foundation of a nuclear domain.
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They identify a significant subset of true lincRNAs that are not translated, including the well-documented RNA element NEAT1.
“We also identify a significant subset of true lincRNAs that are not translated, including the well-documented RNA element NEAT1”
From Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity and Dynamics of Mammalian Proteomes (2011) · cites this paper as Clemson et al., 2009
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- Authors
- Clemson, C. M., Hutchinson, J. N., Sara, S. A., Ensminger, A. W., Fox, A. H., Chess, A., & Lawrence, J. B.
- Affiliations
- University of Massachusetts Chan Medical School (US)
- Harvard University (US)
- The University of Western Australia (AU)
- Corresponding author
- Clemson, Christine M.
- Lawrence, Jeanne B.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 33
- Issue
- 6
- Pages
- 717-726
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC2696186
- OpenAlex
- W2051282896
- Cited by
- 1,445
- References
- 53
- Citations by year
- 2026: 61 · 2025: 62 · 2024: 89 · 2023: 89 · 2022: 101 · 2021: 140 · 2020: 109 · 2019: 111 · 2018: 121 · 2017: 119 · 2016: 100 · 2015: 69 · 2014: 67 · 2013: 76 · 2012: 38
- FWCI
- 17.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion, other-oa)
- Funders
- National Institutes of Health (2r01gm053234-16a1)
- National Institute on Deafness and Other Communication Disorders (DC003263, R01DC003263)
- National Institute of General Medical Sciences (GM068711, R01GM068138, GM 68138, GM-53234, R01 GM-053234, R01GM068711)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007439, HD007439)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- paraspeckles, non-coding RNA, nuclear domains, p54, A-to-I RNA editing, RNA-RNA interactions, RNAi depletion
- MeSH terms
- Animals, Cell Nucleus, Cells, Cultured, Endoribonucleases, Humans, Nuclear Proteins, RNA, Small Nuclear, RNA-Binding Proteins, RNA Interference, Intranuclear Inclusion Bodies, Immunoprecipitation, Mice, Gene Knockdown Techniques, Chloroplast Proteins
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