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Cited by 6Cellular transport and secretionFungal and yeast genetics researchMicrotubule and mitosis dynamics

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An interaction between β′-COP and the ArfGAP, Glo3, maintains post-Golgi cargo recycling

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Xie, Boyang, Guillem, Clara, Date, Swapneeta S., Cohen, Cameron I., Jung, Christian, Kendall, Amy K., Best, Jordan T., Graham, Todd R., Jackson, Lauren P.·2023·Journal of Cell Biology
DOI 10.1083/jcb.202008061PMC PMC9960064

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Abstract

The essential COPI coat mediates retrieval of transmembrane proteins at the Golgi and endosomes following recruitment by the small GTPase, Arf1. ArfGAP proteins regulate COPI coats, but molecular details for COPI recognition by ArfGAPs remain elusive. Biochemical and biophysical data reveal how β′-COP propeller domains directly engage the yeast ArfGAP, Glo3, with a low micromolar binding affinity. Calorimetry data demonstrate that both β′-COP propeller domains are required to bind Glo3. An acidic patch on β′-COP (D437/D450) interacts with Glo3 lysine residues located within the BoCCS (binding of coatomer, cargo, and SNAREs) region. Targeted point mutations in either Glo3 BoCCS or β′-COP abrogate the interaction in vitro, and loss of the β′-COP/Glo3 interaction drives Ste2 missorting to the vacuole and aberrant Golgi morphology in budding yeast. These data suggest that cells require the β′-COP/Glo3 interaction for cargo recycling via endosomes and the TGN, where β′-COP serves as a molecular platform to coordinate binding to multiple proteins, including Glo3, Arf1, and the COPI F-subcomplex.

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Metadata

Authors
Xie, B., Guillem, C., Date, S. S., Cohen, C. I., Jung, C., Kendall, A. K., Best, J. T., Graham, T. R., & Jackson, L. P.
Affiliations
Vanderbilt University (US)
Corresponding author
Jackson, Lauren P.
ORCID
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2023-02-22
Volume
222
Issue
4
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.202008061
PMC
PMC9960064
OpenAlex
W4321492109
Cited by
6
References
2
Citations by year
2026: 4 · 2025: 1 · 2023: 1
FWCI
0.86
Type
journal article
Language
en
Open access
Licence
Locations
  • The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (R35GM119525, R01GM118452, T32GM008320-34)
  • Pew Charitable Trusts
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
beta-COP, ArfGAP, COPI coat, Arf1, protein-protein interaction, endosomes, trans-Golgi network, Golgi morphology, Saccharomyces cerevisiae
MeSH terms
Golgi Apparatus, Saccharomyces cerevisiae, GTPase-Activating Proteins, Coat Protein Complex I, Coatomer Protein, ADP-Ribosylation Factor 1, Saccharomyces cerevisiae Proteins, SNARE Proteins

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