A Simplified Chemostat for the Growth of Mammalian Cells: Characteristics of Cell Growth in Continuous Culture
Open accessAbstract
A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the population remained stabilized for an indefinite period, but at low flow rates, there was sometimes a cyclical variation in population density. The factor limiting growth rate at input rates of approximately 0.2 volumes per day was not the exhaustion of the medium; but in some experiments a non-dialyzable material appeared which inhibited cell growth.
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Chemostat and perfusion systems can maintain environmental conditions and cell growth rates via a continuous dilution of the culture with fresh medium.
“Such systems can maintain environmental conditions and cell growth rates via a continuous dilution of the culture with fresh medium (Cohen and Eagle, 1961).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Cohen and Eagle, 1961
The introduction (p. 467) says continuous fresh-medium input and removal sustain growth in a constant environment. The Results (pp. 469–472) show that dilution rate affects stabilized cell counts and generation time in the mammalian-cell chemostat. The paper does not establish the same claim for perfusion systems, so the citation supports the chemostat portion only. (LLM, conf=0.82)
Experimental — accuracy not yet measuredChemostat and perfusion systems were first described in 1950 for use in bacterial cultures and later adopted for mammalian cells in 1961.
“Chemostat and perfusion systems were first described in 1950 for use in bacterial cultures (Novick and Szilard, 1950a; b) and later adopted for mammalian cells in 1961 (Cohen and Eagle, 1961).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Cohen and Eagle, 1961
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- Authors
- Cohen, E. P., & Eagle, H.
- Affiliations
- National Institutes of Health (US)
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1961-02-01
- Volume
- 113
- Issue
- 2
- Pages
- 467-474
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2137361
- OpenAlex
- W2056213575
- Cited by
- 59
- References
- 8
- Citations by year
- 2022: 1 · 2021: 3 · 2020: 1 · 2017: 1 · 2013: 1
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Keywords
- chemostat, mammalian cell growth, continuous culture, suspension culture, cell density, generation time, flow rate, growth inhibition, cell growth kinetics
- MeSH terms
- Animals, Cell Count, Cell Cycle, Time Factors, Cell Culture Techniques, Tissue Culture Techniques, Cell Proliferation
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